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- W2024191287 abstract "To image VCAM-1 expression in vitro and ex vivo using MRI VCAM-1 is an adhesion molecule that is involved in atherogenesis, restenosis and microvascular obstruction. A fluorescent paramagnetic microparticle (FPM) was conjugated to a monoclonal antibody targeted towards VCAM-1 using the streptavidin-biotin interaction. Human umbilical vein endothelial cells (HUVECS) were grown to confluence. TNF-exposed as well as virgin HUVECS were then exposed to one of the following; non-targeted FPM alone; FPM targeted against VCAM-1; FPM targeted against VCAM-1 along with an antibody that blocks the binding site of VCAM-1. These were allowed to incubate at 37C for 30 minutes before flow cytometry. Aortae from 16 cholesterol-fed rabbits were incised and perfused with; VCAM-1 targeted FPM; non-targeted FPM; or VCAM-1 targeted FPM in the presence of a blocking antibody. After a 20 minute incubation time, the aortae were excised an imaged using a T2* weighted sequence. 10 of the imaged rabbit aortae were sectioned and viewed under a fluorescent microscope. All data expressed as mean+(-)SD. Flow cytometric examination of the HUVECs demonstrate no significant difference in autofluorescence, as measured by mean fluorescence intensity, between virgin and TNF treated HUVECs (17.587+(-)0.110 vs 16.550+(-)0.824, P=0.163). There is a marked increase in green fluorescence in both groups of cells when VCAM-1 targeted FPM was added (26.68+(-)1.61 P=0.01 for TNF treated cells, 21.313+(-) 0.786 P=0.015 for virgin cells), with greater fluorescence in the TNF treated cells (P=0.035). Coadministration of a VCAM-1 blocking antibody abolished the increased fluorescence induced by administration of the targeted FPM (26.68+(-)1.61 vs 19.83+(-)1.10 P=0.009). Administration of targeted FPM targeted against VCAM-1 caused a visually detectable loss of signal in the luminal surface of atherosclerotic plaque in 4 out of 5 rabbits. The remaining 11 rabbit aortae showed no demonstrable loss of signal at the site of plaque formation. Subsequent histological examination confirms the presence of FPMs on the plaque. This study demonstrates the feasibility of imaging VCAM-1 expression using targeted FPM." @default.
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- W2024191287 date "2010-02-01" @default.
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- W2024191287 title "Abstract No. 337: MRI molecular imaging of VCAM-1" @default.
- W2024191287 doi "https://doi.org/10.1016/j.jvir.2009.12.126" @default.
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