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• W2024192712 abstract "Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is increasingly used in the field of carbohydrate characterization. The majority of oligosaccharides can be investigated with only a minimum extent of fragmentation. However, significant but unwanted sulphate loss occurs if the negative ion MALDI mass spectra of sulphated carbohydrates are recorded. We will show here by using differently sulphated oligosaccharides of the chondroitin sulphate (CS) type that this problem can be minimized by recording the positive ion spectra in the presence of the common MALDI matrix DHB (2,5-dihydroxybenzoic acid). Although the sensitivity of the positive ion spectra is reduced in comparison to the negative ion spectra, the reduction of the sulphate loss is a major advantage – particularly regarding the analysis of mixtures." @default.
• W2024192712 created "2016-06-24" @default.
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• W2024192712 date "2012-01-01" @default.
• W2024192712 modified "2023-09-23" @default.
• W2024192712 title "Positive ion MALDI-TOF mass spectra are more suitable than negative ion spectra to characterize sulphated glycosaminoglycan oligosaccharides" @default.
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• W2024192712 doi "https://doi.org/10.1016/j.ijms.2011.11.003" @default.
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