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• W2024199022 abstract "Significance The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been used to generate targeted gene editing in plants. However, it is not known whether CRISPR/Cas-induced gene modifications in plants occur in somatic cells only or whether some or all of the modifications can enter the germ line to become heritable. Through systematic and multigenerational analysis, this study demonstrates that although the majority of gene modifications detected in the first generation CRISPR/Cas transgenic Arabidopsis plants were somatic mutations only, heritable mutations could be found in subsequent generations. In addition, deep sequencing of CRISPR/Cas-modified Arabidopsis genomes did not detect any off-targets. The work demonstrates that the CRISPR/Cas method can effectively create specific gene modifications in planta that are stably transmitted through the germ line to future generations." @default.
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• W2024199022 date "2014-02-18" @default.
• W2024199022 modified "2023-10-11" @default.
• W2024199022 title "Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in <i>Arabidopsis</i>" @default.
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• W2024199022 doi "https://doi.org/10.1073/pnas.1400822111" @default.
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