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• W2024202230 endingPage "10166" @default.
• W2024202230 startingPage "10162" @default.
• W2024202230 abstract "Escherichia coli RNA polymerase (RNAP) alpha subunit serves as the initiator for RNAP assembly, which proceeds according to the pathway 2 alpha-->alpha 2-->alpha 2 beta-->alpha 2 beta beta'-->alpha 2 beta beta' sigma. In this work, we have used hydroxyl-radical protein footprinting to define determinants of alpha for interaction with beta, beta', and sigma. Our results indicate that amino acids 30-75 of alpha are protected from hydroxyl-radical-mediated proteolysis upon interaction with beta (i.e., in alpha 2 beta, alpha 2 beta beta', and alpha 2 beta beta' sigma), and amino acids 175-210 of alpha are protected from hydroxyl-radical-mediated proteolysis upon interaction with beta' (i.e., in alpha 2 beta beta' and alpha 2 beta beta' sigma). The protected regions are conserved in the alpha homologs of prokaryotic, eukaryotic, archaeal, and chloroplast RNAPs and contain sites of substitutions that affect RNAP assembly. We conclude that the protected regions define determinants of alpha for direct functional interaction with beta and beta'. The observed maximal magnitude of protection upon interaction with beta and the observed maximal magnitude of protection upon interaction with beta' both correspond to the expected value for complete protection of one of the two alpha protomers of RNAP (i.e., 50% protection). We propose that only one of the two alpha protomers of RNAP interacts with beta and that only one of the two alpha protomers of RNAP interacts with beta'." @default.
• W2024202230 created "2016-06-24" @default.
• W2024202230 creator A5010090486 @default.
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• W2024202230 creator A5046329493 @default.
• W2024202230 creator A5056880626 @default.
• W2024202230 creator A5087284111 @default.
• W2024202230 date "1996-09-17" @default.
• W2024202230 modified "2023-10-18" @default.
• W2024202230 title "Determinants of RNA polymerase alpha subunit for interaction with beta, beta', and sigma subunits: hydroxyl-radical protein footprinting." @default.
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• W2024202230 doi "https://doi.org/10.1073/pnas.93.19.10162" @default.
• W2024202230 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/38354" @default.
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• W2024202230 hasPublicationYear "1996" @default.
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