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• W2024204024 endingPage "0" @default.
• W2024204024 startingPage "34e" @default.
• W2024204024 abstract "We characterized the fluorescence resonance energy transfer (FRET) from pyrene (donor) to perylene (acceptor) for nucleic acid assays under homo­geneous solution conditions. We used the hybridization between a target 32mer and its complementary two sequential 16mer deoxyribonucleotides whose neighboring terminals were each respectively labeled with a pyrene and a perylene residue. A transfer efficiency of ~100% was attained upon the hybridization when observing perylene fluorescence at 459 nm with 347-nm excitation of a pyrene absorption peak. The Förster distance between two dye residues was 22.3 Å (the orientation factor of 2/3). We could change the distance between the residues by inserting various numbers of nucleotides into the center of the target, thus creating a gap between the dye residues on a hybrid. Assuming that the number of inserted nucleo­tides is proportional to the distance between the dye residues, the energy transfer efficiency versus number of inserted nucleotides strictly obeyed the Förster theory. The mean inter-nucleotide distance of the single-stranded portion was estimated to be 2.1 Å. Comparison between the fluorescent properties of a pyrene–perylene pair with those of a widely used fluorescein–rhodamine pair showed that the pyrene–perylene FRET is suitable for hybridization assays." @default.
• W2024204024 created "2016-06-24" @default.
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• W2024204024 date "2000-04-15" @default.
• W2024204024 modified "2023-09-23" @default.
• W2024204024 title "Fluorescence resonance energy transfer from pyrene to perylene labels for nucleic acid hybridization assays under homogeneous solution conditions" @default.
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• W2024204024 doi "https://doi.org/10.1093/nar/28.8.e34" @default.
• W2024204024 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/102838" @default.
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