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- W2024207645 abstract "MALDI-TOFMS approaches have been developed for high-throughput screening of naturally occurring mixtures of rhamnolipids from Pseudomonas spp. Monorhamnolipids and dirhamnolipids are readily distinguished by characteristic molecular adduct ions, [M+Na](+) and [M-H+Na(2)](+), with variously acylated rhamnolipids differing by 28 mu. Following proton-deuterium exchange, deuterated [M+Na-4(1)H+4(2)H](+) and [M+Na-6(1)H+6(2)H](+) ions are observed for the monorhamnolipids and dirhamnolipids, respectively, which allows rapid identification of these molecules. The described approach has been validated by compositional analysis using GC/MS, fractionation by RPHPLC, and analysis by 1D and 2D NMR spectroscopy. MALDI-TOFMS analysis allows the rapid screening of variously acylated rhamnolipids, and has potential for selective identification of new surfactants from microbial strains." @default.
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- W2024207645 date "2009-01-01" @default.
- W2024207645 modified "2023-09-23" @default.
- W2024207645 title "MALDI-TOF mass spectrometry of naturally occurring mixtures of monorhamnolipids and dirhamnolipids" @default.
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- W2024207645 doi "https://doi.org/10.1016/j.carres.2008.10.013" @default.
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