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- W2024210018 abstract "The acute effects of 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3) on Ca2+ influx in isolated rat enterocytes were studied. The hormone significantly increased 45Ca2+ uptake by the cells within 1–10 min in a specific dose-dependent manner (10−11−10−9M) since 25(OH)D3, and 24,25(OH)2D3 were devoid of activity. The effects of 1,25(OH)2DP3 were mimicked by the Ca2+ channel agonist BAY K8644 and completely abolished by nifedipine (1 μM) and verapamil (10 μM). Incubation of duodenal cells with 1,25(OH)2D3 rapidly (1–5 min) incrased cAMP levels. Forskolin caused a rapid increase in Ca2+ uptake by enterocytes which was similar to the action of the hormone. Moreover, pretreatment of cells with the specific cAMP inhibitor Rp-cAMPS suppressed the changes in 45Ca influx induced by 1,25(OH)2D3. These resuls provide the first evidence involving Ca2+ channel activation through the cAMP pathway by 1,25(OH)2D3 in mammalian intestinal cells." @default.
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- W2024210018 date "1994-03-01" @default.
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- W2024210018 title "Rapid 1,25(OH)2-vitamin D3 stimulation of calcium uptake by rat intestinal cells involves a dihydropyridine-sensitive cAMP-dependent pathway" @default.
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- W2024210018 doi "https://doi.org/10.1016/0898-6568(94)90034-5" @default.
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