Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024214504> ?p ?o ?g. }
- W2024214504 endingPage "50" @default.
- W2024214504 startingPage "42" @default.
- W2024214504 abstract "In spite of their many potential applications, recombinant antibody molecules selected by phage display are rarely available commercially, one reason being the absence of robust bacterial expression systems that yield sufficient quantities of reagents for routine applications. We previously described the construction and validation of an intrabody library that allows the selection of single-chain Fv (scFv) fragments solubly expressed in the cytoplasm. Here, we show that it is possible to obtain monomeric scFvs binding specifically to human papillomavirus type 16 E6 and cellular gankyrin oncoproteins in quantities higher than 0.5 g/L of shake-flask culture in E. coli cytoplasm after auto-induction. In addition, stable bivalent scFvs of increased avidity were produced by tagging the scFvs with the dimeric glutathione-S-transferase enzyme (GST). These minibody-like molecules were further engineered by fusion with green fluorescent protein (GFPuv), leading to high yield of functional bivalent fluorescent antibody fragments. Our results demonstrate that scFvs selected from an intrabody library can be engineered into cost-effective bivalent reagents suitable for many biomedical and industrial applications." @default.
- W2024214504 created "2016-06-24" @default.
- W2024214504 creator A5000442922 @default.
- W2024214504 creator A5056269581 @default.
- W2024214504 creator A5057215421 @default.
- W2024214504 creator A5057718463 @default.
- W2024214504 creator A5061695712 @default.
- W2024214504 creator A5074622303 @default.
- W2024214504 creator A5075382719 @default.
- W2024214504 creator A5085260797 @default.
- W2024214504 date "2011-06-01" @default.
- W2024214504 modified "2023-10-18" @default.
- W2024214504 title "Single-chain Fv fragment antibodies selected from an intrabody library as effective mono- or bivalent reagents for in vitro protein detection" @default.
- W2024214504 cites W1572496212 @default.
- W2024214504 cites W1592842097 @default.
- W2024214504 cites W1606431180 @default.
- W2024214504 cites W1826524642 @default.
- W2024214504 cites W1831319429 @default.
- W2024214504 cites W1986935290 @default.
- W2024214504 cites W1987534225 @default.
- W2024214504 cites W1992017029 @default.
- W2024214504 cites W1997976090 @default.
- W2024214504 cites W1998003752 @default.
- W2024214504 cites W2001631134 @default.
- W2024214504 cites W2009096173 @default.
- W2024214504 cites W2009534236 @default.
- W2024214504 cites W2015285552 @default.
- W2024214504 cites W2018107765 @default.
- W2024214504 cites W2020225303 @default.
- W2024214504 cites W2020368956 @default.
- W2024214504 cites W2020905623 @default.
- W2024214504 cites W2035145745 @default.
- W2024214504 cites W2038608355 @default.
- W2024214504 cites W2042548343 @default.
- W2024214504 cites W2042928804 @default.
- W2024214504 cites W2043330120 @default.
- W2024214504 cites W2047199560 @default.
- W2024214504 cites W2054592480 @default.
- W2024214504 cites W2068861782 @default.
- W2024214504 cites W2073265895 @default.
- W2024214504 cites W2080730448 @default.
- W2024214504 cites W2082245020 @default.
- W2024214504 cites W2085515262 @default.
- W2024214504 cites W2087961972 @default.
- W2024214504 cites W2092803887 @default.
- W2024214504 cites W2099666333 @default.
- W2024214504 cites W2103197465 @default.
- W2024214504 cites W2105270854 @default.
- W2024214504 cites W2106246537 @default.
- W2024214504 cites W2107397967 @default.
- W2024214504 cites W2118338755 @default.
- W2024214504 cites W2152996957 @default.
- W2024214504 cites W2154719936 @default.
- W2024214504 doi "https://doi.org/10.1016/j.jim.2011.04.001" @default.
- W2024214504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21501618" @default.
- W2024214504 hasPublicationYear "2011" @default.
- W2024214504 type Work @default.
- W2024214504 sameAs 2024214504 @default.
- W2024214504 citedByCount "9" @default.
- W2024214504 countsByYear W20242145042013 @default.
- W2024214504 countsByYear W20242145042014 @default.
- W2024214504 countsByYear W20242145042015 @default.
- W2024214504 countsByYear W20242145042021 @default.
- W2024214504 crossrefType "journal-article" @default.
- W2024214504 hasAuthorship W2024214504A5000442922 @default.
- W2024214504 hasAuthorship W2024214504A5056269581 @default.
- W2024214504 hasAuthorship W2024214504A5057215421 @default.
- W2024214504 hasAuthorship W2024214504A5057718463 @default.
- W2024214504 hasAuthorship W2024214504A5061695712 @default.
- W2024214504 hasAuthorship W2024214504A5074622303 @default.
- W2024214504 hasAuthorship W2024214504A5075382719 @default.
- W2024214504 hasAuthorship W2024214504A5085260797 @default.
- W2024214504 hasConcept C104317684 @default.
- W2024214504 hasConcept C123894998 @default.
- W2024214504 hasConcept C142613039 @default.
- W2024214504 hasConcept C147816474 @default.
- W2024214504 hasConcept C153911025 @default.
- W2024214504 hasConcept C159654299 @default.
- W2024214504 hasConcept C178790620 @default.
- W2024214504 hasConcept C179223381 @default.
- W2024214504 hasConcept C181199279 @default.
- W2024214504 hasConcept C185592680 @default.
- W2024214504 hasConcept C186268636 @default.
- W2024214504 hasConcept C190062978 @default.
- W2024214504 hasConcept C202751555 @default.
- W2024214504 hasConcept C21951064 @default.
- W2024214504 hasConcept C2776620830 @default.
- W2024214504 hasConcept C2779281246 @default.
- W2024214504 hasConcept C40767141 @default.
- W2024214504 hasConcept C54355233 @default.
- W2024214504 hasConcept C544153396 @default.
- W2024214504 hasConcept C55493867 @default.
- W2024214504 hasConcept C86803240 @default.
- W2024214504 hasConceptScore W2024214504C104317684 @default.
- W2024214504 hasConceptScore W2024214504C123894998 @default.
- W2024214504 hasConceptScore W2024214504C142613039 @default.
- W2024214504 hasConceptScore W2024214504C147816474 @default.
- W2024214504 hasConceptScore W2024214504C153911025 @default.