FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024223802> ?p ?o ?g. }

• W2024223802 abstract "Abstract Background Escherichia coli synthesizes three membrane-bound molybdenum- and selenocysteine-containing formate dehydrogenases, as well as up to four membrane-bound [NiFe]-hydrogenases. Two of the formate dehydrogenases (Fdh-N and Fdh-O) and two of the hydrogenases (Hyd-1 and Hyd-2) have their respective catalytic subunits located in the periplasm and these enzymes have been shown previously to oxidize formate and hydrogen, respectively, and thus function in energy metabolism. Mutants unable to synthesize the [NiFe]-hydrogenases retain a H 2 : benzyl viologen oxidoreductase activity. The aim of this study was to identify the enzyme or enzymes responsible for this activity. Results Here we report the identification of a new H 2 : benzyl viologen oxidoreductase enzyme activity in E. coli that is independent of the [NiFe]-hydrogenases. This enzyme activity was originally identified after non-denaturing polyacrylamide gel electrophoresis and visualization of hydrogen-oxidizing activity by specific staining. Analysis of a crude extract derived from a variety of E. coli mutants unable to synthesize any [NiFe]-hydrogenase-associated enzyme activity revealed that the mutants retained this specific hydrogen-oxidizing activity. Enrichment of this enzyme activity from solubilised membrane fractions of the hydrogenase-negative mutant FTD147 by ion-exchange, hydrophobic interaction and size-exclusion chromatographies followed by mass spectrometric analysis identified the enzymes Fdh-N and Fdh-O. Analysis of defined mutants devoid of selenocysteine biosynthetic capacity or carrying deletions in the genes encoding the catalytic subunits of Fdh-N and Fdh-O demonstrated that both enzymes catalyze hydrogen activation. Fdh-N and Fdh-O can also transfer the electrons derived from oxidation of hydrogen to other redox dyes. Conclusions The related respiratory molybdo-selenoproteins Fdh-N and Fdh-O of Escherichia coli have hydrogen-oxidizing activity. These findings demonstrate that the energy-conserving selenium- and molybdenum-dependent formate dehydrogenases Fdh-N and Fdh-O exhibit a degree of promiscuity with respect to the electron donor they use and identify a new class of dihydrogen-oxidizing enzyme." @default.
• W2024223802 created "2016-06-24" @default.
• W2024223802 creator A5000337010 @default.
• W2024223802 creator A5032311558 @default.
• W2024223802 creator A5034064941 @default.
• W2024223802 creator A5055325510 @default.
• W2024223802 creator A5069450100 @default.
• W2024223802 creator A5076952708 @default.
• W2024223802 creator A5084158849 @default.
• W2024223802 creator A5086942242 @default.
• W2024223802 creator A5087043351 @default.
• W2024223802 date "2011-08-01" @default.
• W2024223802 modified "2023-10-03" @default.
• W2024223802 title "The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity" @default.
• W2024223802 cites W1481775159 @default.
• W2024223802 cites W1486135496 @default.
• W2024223802 cites W1533336413 @default.
• W2024223802 cites W1548801077 @default.
• W2024223802 cites W1775749144 @default.
• W2024223802 cites W1802996191 @default.
• W2024223802 cites W1848839190 @default.
• W2024223802 cites W1855502384 @default.
• W2024223802 cites W1911705296 @default.
• W2024223802 cites W1960215150 @default.
• W2024223802 cites W1965853158 @default.
• W2024223802 cites W1975105919 @default.
• W2024223802 cites W1977929471 @default.
• W2024223802 cites W1991366673 @default.
• W2024223802 cites W2007400695 @default.
• W2024223802 cites W2010949672 @default.
• W2024223802 cites W2022801286 @default.
• W2024223802 cites W2031215741 @default.
• W2024223802 cites W2042814467 @default.
• W2024223802 cites W2057702599 @default.
• W2024223802 cites W2059369332 @default.
• W2024223802 cites W2081645231 @default.
• W2024223802 cites W2087111834 @default.
• W2024223802 cites W2089600895 @default.
• W2024223802 cites W2101639848 @default.
• W2024223802 cites W2104287309 @default.
• W2024223802 cites W2104684144 @default.
• W2024223802 cites W2112951169 @default.
• W2024223802 cites W2130897733 @default.
• W2024223802 cites W2132358998 @default.
• W2024223802 cites W2140118240 @default.
• W2024223802 cites W2147189708 @default.
• W2024223802 cites W2163813592 @default.
• W2024223802 cites W2166398032 @default.
• W2024223802 cites W2168165683 @default.
• W2024223802 cites W4232999505 @default.
• W2024223802 doi "https://doi.org/10.1186/1471-2180-11-173" @default.
• W2024223802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3160892" @default.
• W2024223802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21806784" @default.
• W2024223802 hasPublicationYear "2011" @default.
• W2024223802 type Work @default.
• W2024223802 sameAs 2024223802 @default.
• W2024223802 citedByCount "54" @default.
• W2024223802 countsByYear W20242238022012 @default.
• W2024223802 countsByYear W20242238022013 @default.
• W2024223802 countsByYear W20242238022014 @default.
• W2024223802 countsByYear W20242238022015 @default.
• W2024223802 countsByYear W20242238022016 @default.
• W2024223802 countsByYear W20242238022017 @default.
• W2024223802 countsByYear W20242238022018 @default.
• W2024223802 countsByYear W20242238022019 @default.
• W2024223802 countsByYear W20242238022020 @default.
• W2024223802 countsByYear W20242238022021 @default.
• W2024223802 countsByYear W20242238022022 @default.
• W2024223802 countsByYear W20242238022023 @default.
• W2024223802 crossrefType "journal-article" @default.
• W2024223802 hasAuthorship W2024223802A5000337010 @default.
• W2024223802 hasAuthorship W2024223802A5032311558 @default.
• W2024223802 hasAuthorship W2024223802A5034064941 @default.
• W2024223802 hasAuthorship W2024223802A5055325510 @default.
• W2024223802 hasAuthorship W2024223802A5069450100 @default.
• W2024223802 hasAuthorship W2024223802A5076952708 @default.
• W2024223802 hasAuthorship W2024223802A5084158849 @default.
• W2024223802 hasAuthorship W2024223802A5086942242 @default.
• W2024223802 hasAuthorship W2024223802A5087043351 @default.
• W2024223802 hasBestOaLocation W20242238021 @default.
• W2024223802 hasConcept C104317684 @default.
• W2024223802 hasConcept C161790260 @default.
• W2024223802 hasConcept C181199279 @default.
• W2024223802 hasConcept C185592680 @default.
• W2024223802 hasConcept C197957613 @default.
• W2024223802 hasConcept C201663137 @default.
• W2024223802 hasConcept C2776087148 @default.
• W2024223802 hasConcept C2778428456 @default.
• W2024223802 hasConcept C2779201268 @default.
• W2024223802 hasConcept C2779268744 @default.
• W2024223802 hasConcept C2780178953 @default.
• W2024223802 hasConcept C547475151 @default.
• W2024223802 hasConcept C55493867 @default.
• W2024223802 hasConcept C79115784 @default.
• W2024223802 hasConcept C86803240 @default.
• W2024223802 hasConceptScore W2024223802C104317684 @default.
• W2024223802 hasConceptScore W2024223802C161790260 @default.
• W2024223802 hasConceptScore W2024223802C181199279 @default.
• W2024223802 hasConceptScore W2024223802C185592680 @default.
• W2024223802 hasConceptScore W2024223802C197957613 @default.

• next