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- W2024225211 abstract "Abstract A simple, rapid and sensitive chemiluminescence method has been developed to measure platelet‐activating factor (PAF). Hydrogen peroxide generated from PAF, upon phospholipase D cleavage, by choline oxidase is determined as chemiluminescence by a luminol‐microperoxidase system. The detection limit of PAF by this method is 5 pmol/tube. The method is reproducible with a 5.5% coefficient of variation at 10 pmol of PAF (n=5). Lipids were extracted from guinea pig neutrophils after stimulation with cytochalasin B and N ‐formyl‐methionyl‐leucylphenylalanine, and PAF was isolated by high‐performance liquid chromatography and determined by chemiluminescence measurements. The amount of PAF detected was 96.1±39.7 (mean ± SD, n=7) pmol/10 8 cells. This highly sensitive method could be useful for the determination of PAF generated under pathophysiological conditions." @default.
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- W2024225211 date "1991-12-01" @default.
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- W2024225211 title "Determination of platelet-activating factor by a chemiluminescence method and its application to stimulated guinea pig neutrophils" @default.
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- W2024225211 doi "https://doi.org/10.1007/bf02536513" @default.
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