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• W2024231569 endingPage "288" @default.
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• W2024231569 abstract "The impact of simulated solar radiation on DNA and the mitigation of DNA-damaging effects by photoreactivation was studied in a cyanobacterium Anabaena variabilis PCC 7937. Cultures were irradiated under 295, 320 and 395 nm cut-off filters as well as seven other filters such as WG 280, WG 295, WG 305, WG 320, WG 335, WG 345 and GG 400. Growth of the test organism was found to be affected mostly under UV-B radiation as compared to PAR and PAR + UV-A radiations. Amplification of 16s rDNA and RAPD profile was significantly affected following exposure of genomic DNA to UV-B radiation. The formation of T<>T CPDs was recorded only in the cultures irradiated with UV-B radiation (i.e., under 295 nm as well as under WG 280, WG 295 and WG 305 nm cut-off filters), but maximum yield was found under 280 nm cut-off filter. Furthermore, the considerable induction of thymine dimers was observed with increasing UV-irradiation times. Fluorometric analysis of DNA unwinding (FADU) assay for UV-induced DNA strand breaks exhibited the maximum loss in the percentage of dsDNA under UV-B radiation followed by UV-A and PAR in comparison to the light control samples. We observed that T<>T CPD repair is light-dependent, since these lesions were more efficiently removed upon exposure to visible light than in the darkness. Blue radiation was found to be the most effective in photoreactivation than any other wavebands of light. Furthermore, the rate of photoreactivation was measured under varying temperatures (10, 20 and 30 °C); the repair rate was found to be the maximum at 20 °C under white fluorescent light. Our results indicate that photoreactivation play an important role in survival of the organism under natural conditions in spite of being exposed to the UV-B component present in the solar drops." @default.
• W2024231569 created "2016-06-24" @default.
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• W2024231569 creator A5046763586 @default.
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• W2024231569 creator A5075622898 @default.
• W2024231569 date "2011-12-01" @default.
• W2024231569 modified "2023-10-16" @default.
• W2024231569 title "Ultraviolet-B-induced DNA damage and photorepair in the cyanobacterium Anabaena variabilis PCC 7937" @default.
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• W2024231569 doi "https://doi.org/10.1016/j.envexpbot.2011.06.010" @default.
• W2024231569 hasPublicationYear "2011" @default.
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