Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024236811> ?p ?o ?g. }
- W2024236811 endingPage "456" @default.
- W2024236811 startingPage "447" @default.
- W2024236811 abstract "Abstract Effects of some detergents—most frequently used in membrane raft studies—on the polymerization properties of actin were examined under in vitro and in vivo conditions, for protein and cellular investigations, respectively. Under in vitro conditions the polymerization rates were measured with pyrene‐labeled actin. We found that polymerization rate depended on the detergent concentration by following either biphasic characteristics or only decreasing tendency. The strongest effects were observed at relatively low detergent concentrations. SDS–PAGE electrophoresis and dynamic light‐scattering measurements provided further evidences for the size distribution of actin filaments formed under the influence of detergents. Comparing the polymerization rates measured in the presence of different detergents to those obtained with various magnesium and KCl concentrations showed that detergents may influence the actin polymerization at three levels by modifying: (i) the monomer–monomer interaction, (ii) the local ionic strength, and (iii) the affinity of actin for various cations. In vivo studies on NIH 3T3MDR1 cells using TRITC‐phalloidin detected fast depolymerization of large extent around the critical micellar concentrations of the detergents. We concluded that microdomain insolubility observed in the presence of detergents is hardly to be the result of the stabilization of the submembrane actin cytoskeleton merely; rather inter‐lipid and lipid–protein interactions are also involved within the detergent‐resistant membranes. © 2010 International Society for Advancement of Cytometry" @default.
- W2024236811 created "2016-06-24" @default.
- W2024236811 creator A5007073460 @default.
- W2024236811 creator A5024653895 @default.
- W2024236811 creator A5061320307 @default.
- W2024236811 creator A5061717417 @default.
- W2024236811 creator A5073332737 @default.
- W2024236811 creator A5086228493 @default.
- W2024236811 date "2010-02-11" @default.
- W2024236811 modified "2023-10-16" @default.
- W2024236811 title "The effects of detergents on the polymerization properties of actin" @default.
- W2024236811 cites W1518097944 @default.
- W2024236811 cites W1538692246 @default.
- W2024236811 cites W1835632918 @default.
- W2024236811 cites W1899282607 @default.
- W2024236811 cites W1991494909 @default.
- W2024236811 cites W1992874837 @default.
- W2024236811 cites W2008917738 @default.
- W2024236811 cites W2009532293 @default.
- W2024236811 cites W2011456649 @default.
- W2024236811 cites W2024141074 @default.
- W2024236811 cites W2024372419 @default.
- W2024236811 cites W2025289160 @default.
- W2024236811 cites W2040558543 @default.
- W2024236811 cites W2044377819 @default.
- W2024236811 cites W2053429769 @default.
- W2024236811 cites W2055054035 @default.
- W2024236811 cites W2057394333 @default.
- W2024236811 cites W2061382220 @default.
- W2024236811 cites W2072546470 @default.
- W2024236811 cites W2077634865 @default.
- W2024236811 cites W2077999459 @default.
- W2024236811 cites W2084103398 @default.
- W2024236811 cites W2088015840 @default.
- W2024236811 cites W2090855399 @default.
- W2024236811 cites W2093731483 @default.
- W2024236811 cites W2097751688 @default.
- W2024236811 cites W2101223910 @default.
- W2024236811 cites W2104527243 @default.
- W2024236811 cites W2108598406 @default.
- W2024236811 cites W2110726109 @default.
- W2024236811 cites W2121936687 @default.
- W2024236811 cites W2125682735 @default.
- W2024236811 cites W2130513806 @default.
- W2024236811 cites W2138507457 @default.
- W2024236811 cites W2139283168 @default.
- W2024236811 cites W2141367941 @default.
- W2024236811 cites W2145367033 @default.
- W2024236811 cites W2151397718 @default.
- W2024236811 cites W2151903342 @default.
- W2024236811 cites W2152804934 @default.
- W2024236811 cites W2153062931 @default.
- W2024236811 cites W2155653297 @default.
- W2024236811 cites W2159719571 @default.
- W2024236811 cites W4239435837 @default.
- W2024236811 cites W4252028749 @default.
- W2024236811 cites W2084645218 @default.
- W2024236811 cites W2152677503 @default.
- W2024236811 doi "https://doi.org/10.1002/cyto.a.20855" @default.
- W2024236811 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20151434" @default.
- W2024236811 hasPublicationYear "2010" @default.
- W2024236811 type Work @default.
- W2024236811 sameAs 2024236811 @default.
- W2024236811 citedByCount "5" @default.
- W2024236811 countsByYear W20242368112017 @default.
- W2024236811 countsByYear W20242368112020 @default.
- W2024236811 countsByYear W20242368112023 @default.
- W2024236811 crossrefType "journal-article" @default.
- W2024236811 hasAuthorship W2024236811A5007073460 @default.
- W2024236811 hasAuthorship W2024236811A5024653895 @default.
- W2024236811 hasAuthorship W2024236811A5061320307 @default.
- W2024236811 hasAuthorship W2024236811A5061717417 @default.
- W2024236811 hasAuthorship W2024236811A5073332737 @default.
- W2024236811 hasAuthorship W2024236811A5086228493 @default.
- W2024236811 hasConcept C11268172 @default.
- W2024236811 hasConcept C12554922 @default.
- W2024236811 hasConcept C125705527 @default.
- W2024236811 hasConcept C127413603 @default.
- W2024236811 hasConcept C130861313 @default.
- W2024236811 hasConcept C142669718 @default.
- W2024236811 hasConcept C14631669 @default.
- W2024236811 hasConcept C1491633281 @default.
- W2024236811 hasConcept C155672457 @default.
- W2024236811 hasConcept C166940927 @default.
- W2024236811 hasConcept C178790620 @default.
- W2024236811 hasConcept C184651966 @default.
- W2024236811 hasConcept C185592680 @default.
- W2024236811 hasConcept C188027245 @default.
- W2024236811 hasConcept C2776861759 @default.
- W2024236811 hasConcept C2993400109 @default.
- W2024236811 hasConcept C41625074 @default.
- W2024236811 hasConcept C42360764 @default.
- W2024236811 hasConcept C43617362 @default.
- W2024236811 hasConcept C44228677 @default.
- W2024236811 hasConcept C49852136 @default.
- W2024236811 hasConcept C521977710 @default.
- W2024236811 hasConcept C55493867 @default.
- W2024236811 hasConcept C86803240 @default.