Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024238013> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2024238013 endingPage "1090" @default.
- W2024238013 startingPage "1083" @default.
- W2024238013 abstract "The conserved residues, Arg-349 and Asp-373, of the renal Na(+)/dicarboxylate cotransporter (NaDC-1) have been shown in our previous studies to affect substrate affinity and cation binding. In this study, amino acids surrounding Arg-349 and Asp-373 were individually mutated to cysteines and their sensitivity to methanethiosulfonate reagents (MTS) was tested. Only three of the 21 mutants were sensitive to MTS reagents: R349C, S372C, and D373C. The R349C mutant had reduced activity which was restored by chemical modification with MTSEA. The effect of MTSEA was only observed in the presence of sodium, indicating that Arg-349 is conformationally accessible. The succinate transport activity of the S372C mutant was stimulated by both MTSEA and MTSET. The D373C mutant was very sensitive to inhibition by MTSET (K(i) = 0.5 microM) in sodium buffer. The inhibition of D373C by MTSET was prevented by substrate, suggesting that the substrate-induced conformational change occludes the residue. We conclude that the accessibility of Arg-349 and Asp-373 is likely to change with the conformational states of the transport cycle." @default.
- W2024238013 created "2016-06-24" @default.
- W2024238013 creator A5010887429 @default.
- W2024238013 creator A5086969739 @default.
- W2024238013 date "2001-12-27" @default.
- W2024238013 modified "2023-10-10" @default.
- W2024238013 title "Arginine-349 and Aspartate-373 of the Na<sup>+</sup>/Dicarboxylate Cotransporter Are Conformationally Sensitive Residues" @default.
- W2024238013 cites W1553933303 @default.
- W2024238013 cites W1707091457 @default.
- W2024238013 cites W1858064435 @default.
- W2024238013 cites W2016562281 @default.
- W2024238013 cites W2054999309 @default.
- W2024238013 cites W2068835083 @default.
- W2024238013 cites W2072772532 @default.
- W2024238013 cites W2081943673 @default.
- W2024238013 cites W2161495721 @default.
- W2024238013 cites W2441654890 @default.
- W2024238013 doi "https://doi.org/10.1021/bi0156761" @default.
- W2024238013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11790133" @default.
- W2024238013 hasPublicationYear "2001" @default.
- W2024238013 type Work @default.
- W2024238013 sameAs 2024238013 @default.
- W2024238013 citedByCount "15" @default.
- W2024238013 countsByYear W20242380132012 @default.
- W2024238013 crossrefType "journal-article" @default.
- W2024238013 hasAuthorship W2024238013A5010887429 @default.
- W2024238013 hasAuthorship W2024238013A5086969739 @default.
- W2024238013 hasConcept C104317684 @default.
- W2024238013 hasConcept C143065580 @default.
- W2024238013 hasConcept C178790620 @default.
- W2024238013 hasConcept C185592680 @default.
- W2024238013 hasConcept C188053792 @default.
- W2024238013 hasConcept C18903297 @default.
- W2024238013 hasConcept C2777289219 @default.
- W2024238013 hasConcept C2777468819 @default.
- W2024238013 hasConcept C2779856020 @default.
- W2024238013 hasConcept C2781338088 @default.
- W2024238013 hasConcept C515207424 @default.
- W2024238013 hasConcept C537181965 @default.
- W2024238013 hasConcept C55493867 @default.
- W2024238013 hasConcept C71240020 @default.
- W2024238013 hasConcept C8010536 @default.
- W2024238013 hasConcept C86803240 @default.
- W2024238013 hasConceptScore W2024238013C104317684 @default.
- W2024238013 hasConceptScore W2024238013C143065580 @default.
- W2024238013 hasConceptScore W2024238013C178790620 @default.
- W2024238013 hasConceptScore W2024238013C185592680 @default.
- W2024238013 hasConceptScore W2024238013C188053792 @default.
- W2024238013 hasConceptScore W2024238013C18903297 @default.
- W2024238013 hasConceptScore W2024238013C2777289219 @default.
- W2024238013 hasConceptScore W2024238013C2777468819 @default.
- W2024238013 hasConceptScore W2024238013C2779856020 @default.
- W2024238013 hasConceptScore W2024238013C2781338088 @default.
- W2024238013 hasConceptScore W2024238013C515207424 @default.
- W2024238013 hasConceptScore W2024238013C537181965 @default.
- W2024238013 hasConceptScore W2024238013C55493867 @default.
- W2024238013 hasConceptScore W2024238013C71240020 @default.
- W2024238013 hasConceptScore W2024238013C8010536 @default.
- W2024238013 hasConceptScore W2024238013C86803240 @default.
- W2024238013 hasIssue "3" @default.
- W2024238013 hasLocation W20242380131 @default.
- W2024238013 hasLocation W20242380132 @default.
- W2024238013 hasOpenAccess W2024238013 @default.
- W2024238013 hasPrimaryLocation W20242380131 @default.
- W2024238013 hasRelatedWork W2007306029 @default.
- W2024238013 hasRelatedWork W2037779842 @default.
- W2024238013 hasRelatedWork W2041386649 @default.
- W2024238013 hasRelatedWork W2045397203 @default.
- W2024238013 hasRelatedWork W2051086962 @default.
- W2024238013 hasRelatedWork W2059389639 @default.
- W2024238013 hasRelatedWork W2068204432 @default.
- W2024238013 hasRelatedWork W2070882743 @default.
- W2024238013 hasRelatedWork W2264338901 @default.
- W2024238013 hasRelatedWork W2395684592 @default.
- W2024238013 hasVolume "41" @default.
- W2024238013 isParatext "false" @default.
- W2024238013 isRetracted "false" @default.
- W2024238013 magId "2024238013" @default.
- W2024238013 workType "article" @default.