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- W2024248896 abstract "In shadowed electron micrographs, bacteriophages φX174 and S13 appear to consist of twelve “knobs” arranged as at the vertices of an icosahedron. The existence of these “knobs” is confirmed by the appearance of both positively and negatively stained preparations of φX174. On heating or treatment with concentrated acetate or urea, φX174 extrudes strands up to 1·3μ in length. The strand diameter may range from less than 10 Å to 40 Å or above. The strands are disrupted by DNase or by heating to 80°C. They are believed to consist of DNA, irregularly coated by protein. Purified φX174 DNA, like other single-stranded nucleic acids, appears as inhomogeneous strands or “random coils” in electron micrographs. A model is proposed for the structure of φX174 in which the bulk of the phage consists of deoxyribonucleoprotein. The configuration of the DNA is thus maintained by direct combination with about 80% of the total protein. The remainder is divided into sixty identical units which are arranged in groups of five to form the twelve “knobs” of the icosahedrally symmetrical outer protein shell." @default.
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- W2024248896 date "1962-03-01" @default.
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- W2024248896 title "Studies on bacteriophage φX174 and its DNA by electron microscopy" @default.
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- W2024248896 doi "https://doi.org/10.1016/s0022-2836(62)80049-7" @default.
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