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- W2024252704 abstract "Mesoporous silica nanoparticles (MSNs) have received intensive recent interest as multi-functional nanocarriers for biological cells and tissues. Their cell uptake and intracellular distribution depends on the surface charge they carry. We develop a new approach for monitoring their uptake and intracellular localization by measuring the local pH value of the MSNs. We synthesized a ratiometric pH-sensor loaded with mesoporous silica nanoparticles that can map the location of the MSN through the site pH in HeLa cells. Positively charged MSN-TA was found to locate in higher pH regions which are identified as cytosol mostly. On the other hand, negatively charged MSN-PPs were trapped in acidic endosomes. Evidence is given for direct penetration of membranes through a charge-mediated membrane–nanoparticle interaction mechanism." @default.
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- W2024252704 date "2012-01-01" @default.
- W2024252704 modified "2023-09-23" @default.
- W2024252704 title "Surface charge effect in intracellular localization of mesoporous silicananoparticles as probed by fluorescent ratiometric pH imaging" @default.
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- W2024252704 doi "https://doi.org/10.1039/c1ra00586c" @default.
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