Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024253318> ?p ?o ?g. }
- W2024253318 endingPage "e1004292" @default.
- W2024253318 startingPage "e1004292" @default.
- W2024253318 abstract "Cryptococcus neoformans encounters a low oxygen environment when it enters the human host. Here, we show that the conserved Ras1 (a small GTPase) and Cdc24 (the guanine nucleotide exchange factor for Cdc42) play an essential role in cryptococcal growth in hypoxia. Suppressor studies indicate that PTP3 functions epistatically downstream of both RAS1 and CDC24 in regulating hypoxic growth. Ptp3 shares sequence similarity to the family of phosphotyrosine-specific protein phosphatases and the ptp3Δ strain failed to grow in 1% O2. We demonstrate that RAS1, CDC24 and PTP3 function in parallel to regulate thermal tolerance but RAS1 and CDC24 function linearly in regulating hypoxic growth while CDC24 and PTP3 reside in compensatory pathways. The ras1Δ and cdc24Δ strains ceased to grow at 1% O2 and became enlarged but viable single cells. Actin polarization in these cells, however, was normal for up to eight hours after transferring to hypoxic conditions. Double deletions of the genes encoding Rho GTPase Cdc42 and Cdc420, but not of the genes encoding Rac1 and Rac2, caused a slight growth retardation in hypoxia. Furthermore, growth in hypoxia was not affected by the deletion of several central genes functioning in the pathways of cAMP, Hog1, or the two-component like phosphorylation system that are critical in the cryptococcal response to osmotic and genotoxic stresses. Interestingly, although deletion of HOG1 rescued the hypoxic growth defect of ras1Δ, cdc24Δ, and ptp3Δ, Hog1 was not hyperphosphorylated in these three mutants in hypoxic conditions. RNA sequencing analysis indicated that RAS1, CDC24 and PTP3 acted upon the expression of genes involved in ergosterol biosynthesis, chromosome organization, RNA processing and protein translation. Moreover, growth of the wild-type strain under low oxygen conditions was affected by sub-inhibitory concentrations of the compounds that inhibit these biological processes, demonstrating the importance of these biological processes in the cryptococcal hypoxia response." @default.
- W2024253318 created "2016-06-24" @default.
- W2024253318 creator A5011214784 @default.
- W2024253318 creator A5025807967 @default.
- W2024253318 creator A5038813367 @default.
- W2024253318 creator A5064575184 @default.
- W2024253318 creator A5068056560 @default.
- W2024253318 creator A5071899808 @default.
- W2024253318 date "2014-04-24" @default.
- W2024253318 modified "2023-10-12" @default.
- W2024253318 title "Molecular Mechanisms of Hypoxic Responses via Unique Roles of Ras1, Cdc24 and Ptp3 in a Human Fungal Pathogen Cryptococcus neoformans" @default.
- W2024253318 cites W1812390765 @default.
- W2024253318 cites W1872803163 @default.
- W2024253318 cites W1898443428 @default.
- W2024253318 cites W1944361623 @default.
- W2024253318 cites W1965248545 @default.
- W2024253318 cites W1968272833 @default.
- W2024253318 cites W1969918683 @default.
- W2024253318 cites W1970417127 @default.
- W2024253318 cites W1973988915 @default.
- W2024253318 cites W1975165380 @default.
- W2024253318 cites W1981228379 @default.
- W2024253318 cites W1985692664 @default.
- W2024253318 cites W1995127340 @default.
- W2024253318 cites W1998006710 @default.
- W2024253318 cites W1999441881 @default.
- W2024253318 cites W2000282782 @default.
- W2024253318 cites W2009235369 @default.
- W2024253318 cites W2022768385 @default.
- W2024253318 cites W2024111969 @default.
- W2024253318 cites W2029192414 @default.
- W2024253318 cites W2033897927 @default.
- W2024253318 cites W2040318628 @default.
- W2024253318 cites W2052789908 @default.
- W2024253318 cites W2054696920 @default.
- W2024253318 cites W2061879227 @default.
- W2024253318 cites W2063436167 @default.
- W2024253318 cites W2071006062 @default.
- W2024253318 cites W2071041831 @default.
- W2024253318 cites W2071818252 @default.
- W2024253318 cites W2072887494 @default.
- W2024253318 cites W2078807391 @default.
- W2024253318 cites W2080158576 @default.
- W2024253318 cites W2087625457 @default.
- W2024253318 cites W2095010637 @default.
- W2024253318 cites W2095032938 @default.
- W2024253318 cites W2096083625 @default.
- W2024253318 cites W2096791952 @default.
- W2024253318 cites W2098117830 @default.
- W2024253318 cites W2100772783 @default.
- W2024253318 cites W2115978411 @default.
- W2024253318 cites W2116627896 @default.
- W2024253318 cites W2120425861 @default.
- W2024253318 cites W2122322808 @default.
- W2024253318 cites W2122683221 @default.
- W2024253318 cites W2122798106 @default.
- W2024253318 cites W2126476309 @default.
- W2024253318 cites W2128881595 @default.
- W2024253318 cites W2130590948 @default.
- W2024253318 cites W2131508801 @default.
- W2024253318 cites W2136616400 @default.
- W2024253318 cites W2137475948 @default.
- W2024253318 cites W2141332553 @default.
- W2024253318 cites W2141458291 @default.
- W2024253318 cites W2147772275 @default.
- W2024253318 cites W2150578582 @default.
- W2024253318 cites W2150919198 @default.
- W2024253318 cites W2154555878 @default.
- W2024253318 cites W2158217645 @default.
- W2024253318 cites W2158645874 @default.
- W2024253318 cites W2162049443 @default.
- W2024253318 cites W2166272926 @default.
- W2024253318 cites W2167785685 @default.
- W2024253318 cites W2168344348 @default.
- W2024253318 cites W2170662254 @default.
- W2024253318 cites W2172047190 @default.
- W2024253318 cites W2234646024 @default.
- W2024253318 cites W4235535738 @default.
- W2024253318 cites W75730325 @default.
- W2024253318 doi "https://doi.org/10.1371/journal.pgen.1004292" @default.
- W2024253318 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3998916" @default.
- W2024253318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24762475" @default.
- W2024253318 hasPublicationYear "2014" @default.
- W2024253318 type Work @default.
- W2024253318 sameAs 2024253318 @default.
- W2024253318 citedByCount "14" @default.
- W2024253318 countsByYear W20242533182015 @default.
- W2024253318 countsByYear W20242533182017 @default.
- W2024253318 countsByYear W20242533182018 @default.
- W2024253318 countsByYear W20242533182019 @default.
- W2024253318 countsByYear W20242533182020 @default.
- W2024253318 countsByYear W20242533182021 @default.
- W2024253318 countsByYear W20242533182022 @default.
- W2024253318 crossrefType "journal-article" @default.
- W2024253318 hasAuthorship W2024253318A5011214784 @default.
- W2024253318 hasAuthorship W2024253318A5025807967 @default.
- W2024253318 hasAuthorship W2024253318A5038813367 @default.
- W2024253318 hasAuthorship W2024253318A5064575184 @default.