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• W2024253573 abstract "PURPOSE: Exercise influences immunity by mobilizing a wide range of leukocytes into the circulation and altering circulating levels of stress and inflammatory factors. How these distinct mechanisms interact is not yet known. We wondered whether cytokine production from a commercially available cell-model of t-lymphocytes, the Jurkat line, would be altered when cultured in the presence of human sera from resting and exercing individuals. METHODS: Serum from 10 healthy male adults (mean age=24.8 yrs ± 0.9), was collected at pre-exercise (PRE) and after (PEAK) a constant work rate 30-min exercise bout on cycle ergometry above the subjects' lactate threshold. The human Jurkat cell line was purchase from ATCC and maintained in RPMI-1640 with 10% FCS. Cells were starved of serum for 48 hours prior to use. Cells were seeded at 5x105 cells/ml in RPMI-1640 supplemented with 25% human serum. Supernatants were harvested at 1 hour and 6 hour, and tested for the presence of interleukin-2 (IL-2), transforming growth factor-β (TGF-β), IL-1 RA (receptor antagonist) and TNF-α, using commercially available Elisa kits. RESULTS: Cells were 100% viable using tryphan-blue at the onset of the experiment and showed no signs of apoptosis in culture. We were able to detect IL-2, TGF-β, TNF-α, and IL-1RA within the acceptable limits for each Elisa. TGF-β increased significantly between 1- and 6-hours when cultured with both PRE and PEAK sera, but there was no difference in the increase between PRE and PEAK sera. A similar result was obtained for TNF-α. IL-1RA was detected in culture, but did not change between 1- and 6-hours for either PRE or PEAK sera. A significant difference was observed in the production of IL-2 such that in PRE sera, IL-2 decreased significantly from 8.2±1.7 pg/ml (SEM) at 1-hour 2.9±1.6 at 6 hours (p <0.017) but there was no difference in IL-2 values when the cells were cultured with PEAK sera (5.8±0.8 at 1-hour; 6.7±2.2). CONCLUSIONS: In these pilot studies, we demonstrated an effect of sera from exercising subject on cytokine production in commercially available cellular models of t-lymphocyte function. In resting sera, the production of IL-2 seems to be attenuated. IL-2 is indicative of a Th-1 type response and has been associated with t-cell activation and inflammation. Sera obtained immediately following heavy exercise mitigated this response, and, therefore, could be construed to be permissive of inflammatory cytokine production from these cells. Whether exercise associated circulating, immuno-active cytokines like IL-6 play a role in these effects remains unknown" @default.
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• W2024253573 date "2006-05-01" @default.
• W2024253573 modified "2023-09-26" @default.
• W2024253573 title "Effects of Exercise Serum Factors on Jurkat T-cell Cytokines" @default.
• W2024253573 doi "https://doi.org/10.1249/00005768-200605001-02195" @default.
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