FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024255048> ?p ?o ?g. }

• W2024255048 abstract "Neutron diffraction data have been collected to 2.2 Angstrom resolution from a small (0.15 mm(3)) crystal of perdeuterated human aldose reductase (h-AR; MW = 36 kDa) in order to help to determine the protonation state of the enzyme. h-AR belongs to the aldo-keto reductase family and is implicated in diabetic complications. Its ternary complexes (h-AR-coenzyme NADPH-selected inhibitor) provide a good model to study both the enzymatic mechanism and inhibition. Here, the successful production of fully deuterated human aldose reductase [h-AR(D)], subsequent crystallization of the ternary complex h-AR(D)-NADPH-IDD594 and neutron Laue data collection at the LADI instrument at ILL using a crystal volume of just 0.15 mm(3) are reported. Neutron data were recorded to 2 Angstrom resolution, with subsequent data analysis using data to 2.2 Angstrom. This is the first fully deuterated enzyme of this size (36 kDa) to be solved by neutron diffraction and represents a milestone in the field, as the crystal volume is at least one order of magnitude smaller than those usually required for other high-resolution neutron structures determined to date. This illustrates the significant increase in the signal-to-noise ratio of data collected from perdeuterated crystals and demonstrates that good-quality neutron data can now be collected from more typical protein crystal volumes. Indeed, the signal-to-noise ratio is then dominated by other sources of instrument background, the nature of which is under investigation. This is important for the design of future instruments, which should take maximum advantage of the reduction in the intrinsic diffraction pattern background from fully deuterated samples." @default.
• W2024255048 created "2016-06-24" @default.
• W2024255048 creator A5010920048 @default.
• W2024255048 creator A5011183452 @default.
• W2024255048 creator A5013467567 @default.
• W2024255048 creator A5018333936 @default.
• W2024255048 creator A5032506841 @default.
• W2024255048 creator A5043431406 @default.
• W2024255048 creator A5076922889 @default.
• W2024255048 creator A5078892637 @default.
• W2024255048 creator A5090716639 @default.
• W2024255048 date "2005-09-28" @default.
• W2024255048 modified "2023-09-27" @default.
• W2024255048 title "High-resolution neutron protein crystallography with radically small crystal volumes: application of perdeuteration to human aldose reductase" @default.
• W2024255048 cites W2003792985 @default.
• W2024255048 cites W2022921932 @default.
• W2024255048 cites W2027103061 @default.
• W2024255048 cites W2028495056 @default.
• W2024255048 cites W2036765328 @default.
• W2024255048 cites W2039945385 @default.
• W2024255048 cites W2056632222 @default.
• W2024255048 cites W2063059538 @default.
• W2024255048 cites W2072517025 @default.
• W2024255048 cites W2094536143 @default.
• W2024255048 cites W2129475394 @default.
• W2024255048 cites W2131942336 @default.
• W2024255048 cites W2166976731 @default.
• W2024255048 cites W2171746911 @default.
• W2024255048 cites W2027435936 @default.
• W2024255048 doi "https://doi.org/10.1107/s0907444905024285" @default.
• W2024255048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16204895" @default.
• W2024255048 hasPublicationYear "2005" @default.
• W2024255048 type Work @default.
• W2024255048 sameAs 2024255048 @default.
• W2024255048 citedByCount "54" @default.
• W2024255048 countsByYear W20242550482013 @default.
• W2024255048 countsByYear W20242550482014 @default.
• W2024255048 countsByYear W20242550482015 @default.
• W2024255048 countsByYear W20242550482016 @default.
• W2024255048 countsByYear W20242550482017 @default.
• W2024255048 countsByYear W20242550482018 @default.
• W2024255048 countsByYear W20242550482019 @default.
• W2024255048 countsByYear W20242550482020 @default.
• W2024255048 countsByYear W20242550482021 @default.
• W2024255048 countsByYear W20242550482022 @default.
• W2024255048 countsByYear W20242550482023 @default.
• W2024255048 crossrefType "journal-article" @default.
• W2024255048 hasAuthorship W2024255048A5010920048 @default.
• W2024255048 hasAuthorship W2024255048A5011183452 @default.
• W2024255048 hasAuthorship W2024255048A5013467567 @default.
• W2024255048 hasAuthorship W2024255048A5018333936 @default.
• W2024255048 hasAuthorship W2024255048A5032506841 @default.
• W2024255048 hasAuthorship W2024255048A5043431406 @default.
• W2024255048 hasAuthorship W2024255048A5076922889 @default.
• W2024255048 hasAuthorship W2024255048A5078892637 @default.
• W2024255048 hasAuthorship W2024255048A5090716639 @default.
• W2024255048 hasConcept C115624301 @default.
• W2024255048 hasConcept C121332964 @default.
• W2024255048 hasConcept C152568617 @default.
• W2024255048 hasConcept C153064111 @default.
• W2024255048 hasConcept C178790620 @default.
• W2024255048 hasConcept C181199279 @default.
• W2024255048 hasConcept C185544564 @default.
• W2024255048 hasConcept C185592680 @default.
• W2024255048 hasConcept C199360897 @default.
• W2024255048 hasConcept C203036418 @default.
• W2024255048 hasConcept C2776439734 @default.
• W2024255048 hasConcept C2781285689 @default.
• W2024255048 hasConcept C41008148 @default.
• W2024255048 hasConcept C55493867 @default.
• W2024255048 hasConcept C64452783 @default.
• W2024255048 hasConcept C73585123 @default.
• W2024255048 hasConcept C8010536 @default.
• W2024255048 hasConceptScore W2024255048C115624301 @default.
• W2024255048 hasConceptScore W2024255048C121332964 @default.
• W2024255048 hasConceptScore W2024255048C152568617 @default.
• W2024255048 hasConceptScore W2024255048C153064111 @default.
• W2024255048 hasConceptScore W2024255048C178790620 @default.
• W2024255048 hasConceptScore W2024255048C181199279 @default.
• W2024255048 hasConceptScore W2024255048C185544564 @default.
• W2024255048 hasConceptScore W2024255048C185592680 @default.
• W2024255048 hasConceptScore W2024255048C199360897 @default.
• W2024255048 hasConceptScore W2024255048C203036418 @default.
• W2024255048 hasConceptScore W2024255048C2776439734 @default.
• W2024255048 hasConceptScore W2024255048C2781285689 @default.
• W2024255048 hasConceptScore W2024255048C41008148 @default.
• W2024255048 hasConceptScore W2024255048C55493867 @default.
• W2024255048 hasConceptScore W2024255048C64452783 @default.
• W2024255048 hasConceptScore W2024255048C73585123 @default.
• W2024255048 hasConceptScore W2024255048C8010536 @default.
• W2024255048 hasLocation W20242550481 @default.
• W2024255048 hasLocation W20242550482 @default.
• W2024255048 hasLocation W20242550483 @default.
• W2024255048 hasLocation W20242550484 @default.
• W2024255048 hasOpenAccess W2024255048 @default.
• W2024255048 hasPrimaryLocation W20242550481 @default.
• W2024255048 hasRelatedWork W1977713265 @default.
• W2024255048 hasRelatedWork W2001183907 @default.
• W2024255048 hasRelatedWork W2039260816 @default.
• W2024255048 hasRelatedWork W2048331902 @default.

• next