FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024258033> ?p ?o ?g. }

• W2024258033 endingPage "807" @default.
• W2024258033 startingPage "797" @default.
• W2024258033 abstract "Lot6p (EC 1.5.1.39; Ylr011wp) is the sole quinone oxidoreductase in the budding yeast, Saccharomyces cerevisiae. Using hexahistidine tagged, recombinant Lot6p, we determined the steady-state enzyme kinetic parameters with both NADH and NADPH as electron donors; no cooperativity was observed with these substrates. The NQO1 inhibitor curcumin, the NQO2 inhibitor resveratrol, the bacterial nitroreductase inhibitor nicotinamide and the phosphate mimic vanadate all stabilise the enzyme towards thermal denaturation as judged by differential scanning fluorimetry. All except vanadate have no observable effect on the chemical cross-linking of the two subunits of the Lot6p dimer. These compounds all inhibit Lot6p's oxidoreductase activity, and all except nicotinamide exhibit negative cooperativity. Molecular modelling suggests that curcumin, resveratrol and nicotinamide all bind over the isoalloxazine ring of the FMN cofactor in Lot6p. Resveratrol was predicted to contact an α-helix that links the two active sites. Mutation of Gly-142 (which forms part of this helix) to serine does not greatly affect the thermal stability of the enzyme. However, this variant shows less cooperativity towards resveratrol than the wild type. This suggests a plausible hypothesis for the transmission of information between the subunits and, thus, the molecular mechanism of negative cooperativity in Lot6p. We demonstrate that the quinone oxidoreductase Lot6p shows negative cooperativity towards some inhibitors and explain the molecular basis for this." @default.
• W2024258033 created "2016-06-24" @default.
• W2024258033 creator A5007546946 @default.
• W2024258033 creator A5045038740 @default.
• W2024258033 creator A5052068472 @default.
• W2024258033 date "2014-06-19" @default.
• W2024258033 modified "2023-10-16" @default.
• W2024258033 title "TheSaccharomyces cerevisiaequinone oxidoreductase Lot6p: stability, inhibition and cooperativity" @default.
• W2024258033 cites W1170854545 @default.
• W2024258033 cites W1489452981 @default.
• W2024258033 cites W1503552721 @default.
• W2024258033 cites W1523565294 @default.
• W2024258033 cites W1569233292 @default.
• W2024258033 cites W1885778179 @default.
• W2024258033 cites W1969279038 @default.
• W2024258033 cites W1975368052 @default.
• W2024258033 cites W1976652162 @default.
• W2024258033 cites W1980490891 @default.
• W2024258033 cites W1983457087 @default.
• W2024258033 cites W1990927292 @default.
• W2024258033 cites W1992551518 @default.
• W2024258033 cites W1999919593 @default.
• W2024258033 cites W2003894821 @default.
• W2024258033 cites W2005474992 @default.
• W2024258033 cites W2018922462 @default.
• W2024258033 cites W2020247473 @default.
• W2024258033 cites W2021234742 @default.
• W2024258033 cites W2022582116 @default.
• W2024258033 cites W2023578932 @default.
• W2024258033 cites W2026822031 @default.
• W2024258033 cites W2030313200 @default.
• W2024258033 cites W2031658499 @default.
• W2024258033 cites W2034217426 @default.
• W2024258033 cites W2042086711 @default.
• W2024258033 cites W2048817221 @default.
• W2024258033 cites W2050240238 @default.
• W2024258033 cites W2051452518 @default.
• W2024258033 cites W2056650214 @default.
• W2024258033 cites W2060713967 @default.
• W2024258033 cites W2063398237 @default.
• W2024258033 cites W2065258625 @default.
• W2024258033 cites W2068961740 @default.
• W2024258033 cites W2069764626 @default.
• W2024258033 cites W2073189402 @default.
• W2024258033 cites W2078883578 @default.
• W2024258033 cites W2087070363 @default.
• W2024258033 cites W2093450012 @default.
• W2024258033 cites W2094165517 @default.
• W2024258033 cites W2096004249 @default.
• W2024258033 cites W2108627006 @default.
• W2024258033 cites W2110041043 @default.
• W2024258033 cites W2111210558 @default.
• W2024258033 cites W2121152339 @default.
• W2024258033 cites W2123510974 @default.
• W2024258033 cites W2125652910 @default.
• W2024258033 cites W2129016752 @default.
• W2024258033 cites W2132391006 @default.
• W2024258033 cites W2132595034 @default.
• W2024258033 cites W2134271449 @default.
• W2024258033 cites W2138533612 @default.
• W2024258033 cites W2140792027 @default.
• W2024258033 cites W2147007028 @default.
• W2024258033 cites W2147119806 @default.
• W2024258033 cites W2148385534 @default.
• W2024258033 cites W2150757038 @default.
• W2024258033 cites W2162338692 @default.
• W2024258033 cites W2162723682 @default.
• W2024258033 cites W2163921018 @default.
• W2024258033 cites W2171532556 @default.
• W2024258033 cites W2418361432 @default.
• W2024258033 cites W4293247451 @default.
• W2024258033 cites W56597541 @default.
• W2024258033 doi "https://doi.org/10.1111/1567-1364.12167" @default.
• W2024258033 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24866129" @default.
• W2024258033 hasPublicationYear "2014" @default.
• W2024258033 type Work @default.
• W2024258033 sameAs 2024258033 @default.
• W2024258033 citedByCount "9" @default.
• W2024258033 countsByYear W20242580332014 @default.
• W2024258033 countsByYear W20242580332017 @default.
• W2024258033 countsByYear W20242580332019 @default.
• W2024258033 crossrefType "journal-article" @default.
• W2024258033 hasAuthorship W2024258033A5007546946 @default.
• W2024258033 hasAuthorship W2024258033A5045038740 @default.
• W2024258033 hasAuthorship W2024258033A5052068472 @default.
• W2024258033 hasBestOaLocation W20242580331 @default.
• W2024258033 hasConcept C166014724 @default.
• W2024258033 hasConcept C181199279 @default.
• W2024258033 hasConcept C185592680 @default.
• W2024258033 hasConcept C197957613 @default.
• W2024258033 hasConcept C2779268744 @default.
• W2024258033 hasConcept C55493867 @default.
• W2024258033 hasConcept C71240020 @default.
• W2024258033 hasConcept C86803240 @default.
• W2024258033 hasConceptScore W2024258033C166014724 @default.
• W2024258033 hasConceptScore W2024258033C181199279 @default.
• W2024258033 hasConceptScore W2024258033C185592680 @default.
• W2024258033 hasConceptScore W2024258033C197957613 @default.

• next