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• W2024259851 abstract "Marek's disease virus (MDV) encodes a ribonucleotide reductase (RR), a key regulatory enzyme in the DNA synthesis pathway. The gene coding for the RR of MDV is located in the unique long (UL) region of the genome. The large subunit is encoded by UL39 (RR1) and is predicted to comprise 860 amino acids whereas the small subunit encoded by UL40 (RR2) is predicted to be 343 amino acids long. Immunoprecipitation analysis of MDV-1 (GA strain)-infected cells with T81, a monoclonal antibody specific for RR of MDV, identified two major proteins of 90,000 and 40,000 daltons, corresponding to RR1 and RR2, respectively. In addition, RR was abundantly expressed in the cytoplasm of cells infected with 51 strains of MDV belonging to MDV serotypes 1, 2, and 3 as demonstrated by immunofluorescence staining. Northern blot analysis of RNA extracted from MDV-infected cells showed a major band of around 4.4 kb in size corresponding to the RR1 and RR2 transcripts. In vivo, RR was abundantly expressed in lymphoid organs and feather follicle epithelium of MDV-infected chickens during early cytolytic infection, as determined by immunohistochemistry. There was, however, no expression of RR in MDV-induced tumors in lymphoid organs. The abundant expression of RR in MDV-infected chicken may suggest an important role of RR in the conversion of ribonucleotides to deoxyribonucleotides for MDV DNA synthesis.Identificación y caracterización in vitro de la ribonucleótido reductasa codificada por el virus de Marek.El virus de la enfermedad de Marek (MDV) codifica una enzima ribonucleótido reductasa (RR), que es una enzima reguladora clave en la ruta de la síntesis de ADN. El gene que codifica para la ribonucleótido reductasa del virus de Marek se encuentra en la región única larga (UL) del genoma. La subunidad grande está codificada por UL39 (RR1) y se predice que comprende 860 aminoácidos mientras que la subunidad pequeña codificada por UL40 (RR2) que se prevé que tiene 343 aminoácidos de longitud. El análisis por inmunoprecipitación de células infectadas por el virus de Marek serotipo 1 (cepa GA) utilizando el anticuerpo monoclonal T81, que es específico para la ribonucleótido reductasa del virus de Marek, identificó dos proteínas principales de 90,000 y 40,000 daltons, que corresponden a las RR1 y RR2, respectivamente. Además, la ribonucleótido reductasa fue expresada abundantemente en el citoplasma de las células infectadas con 51 cepas del virus de Marek pertenecientes a los serotipos de MDV 1, 2 y 3 como se demostró por la tinción de inmunofluorescencia. El análisis de transferencia Northern con ARN extraído de células infectadas con el virus de Marek mostraron una banda principal de alrededor de 4.4 Kb de tamaño correspondientes a los transcriptos de RR1 y RR2. La ribonucleótido reductasa fue expresada abundantemente in vivo en órganos linfoides y en el epitelio folículo de la pluma de pollos infectados con el virus de Marek durante la infección citolítica temprana, tal como se determinó por inmunohistoquímica. Sin embargo, no hubo expresión de la ribonucleótido reductasa en tumores de órganos linfoides inducida por el virus de Marek. La expresión abundante de la ribonucleótido reductasa en pollos infectados con el virus de Marek puede sugerir un papel importante de esta enzima en la conversión de ribonucleótidos a desoxirribonucleótidos para la síntesis de ADN de este virus." @default.
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• W2024259851 date "2013-06-01" @default.
• W2024259851 modified "2023-10-17" @default.
• W2024259851 title "Identification and<i>In Vitro</i>Characterization of a Marek's Disease Virus–Encoded Ribonucleotide Reductase" @default.
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• W2024259851 doi "https://doi.org/10.1637/10329-082112-reg.1" @default.
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