FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024272267> ?p ?o ?g. }

• W2024272267 endingPage "12875" @default.
• W2024272267 startingPage "12863" @default.
• W2024272267 abstract "Analysis of the time-dependent behavior of a signaling system can provide insight into its dynamic properties. We employed the nucleocytoplasmic shuttling of the transcriptional repressor Mig1 as readout to characterize Snf1-Mig1 dynamics in single yeast cells. Mig1 binds to promoters of target genes and mediates glucose repression. Mig1 is predominantly located in the nucleus when glucose is abundant. Upon glucose depletion, Mig1 is phosphorylated by the yeast AMP-activated kinase Snf1 and exported into the cytoplasm. We used a three-channel microfluidic device to establish a high degree of control over the glucose concentration exposed to cells. Following regimes of glucose up- and downshifts, we observed a very rapid response reaching a new steady state within less than 1 min, different glucose threshold concentrations depending on glucose up- or downshifts, a graded profile with increased cell-to-cell variation at threshold glucose concentrations, and biphasic behavior with a transient translocation of Mig1 upon the shift from high to intermediate glucose concentrations. Fluorescence loss in photobleaching and fluorescence recovery after photobleaching data demonstrate that Mig1 shuttles constantly between the nucleus and cytoplasm, although with different rates, depending on the presence of glucose. Taken together, our data suggest that the Snf1-Mig1 system has the ability to monitor glucose concentration changes as well as absolute glucose levels. The sensitivity over a wide range of glucose levels and different glucose concentration-dependent response profiles are likely determined by the close integration of signaling with the metabolism and may provide for a highly flexible and fast adaptation to an altered nutritional status." @default.
• W2024272267 created "2016-06-24" @default.
• W2024272267 creator A5008926411 @default.
• W2024272267 creator A5016966501 @default.
• W2024272267 creator A5026743647 @default.
• W2024272267 creator A5045325794 @default.
• W2024272267 creator A5045963974 @default.
• W2024272267 creator A5051529023 @default.
• W2024272267 creator A5076898649 @default.
• W2024272267 creator A5079347888 @default.
• W2024272267 date "2014-05-01" @default.
• W2024272267 modified "2023-10-13" @default.
• W2024272267 title "Yeast AMP-activated Protein Kinase Monitors Glucose Concentration Changes and Absolute Glucose Levels" @default.
• W2024272267 cites W1567086547 @default.
• W2024272267 cites W1572786779 @default.
• W2024272267 cites W1582955199 @default.
• W2024272267 cites W1599051410 @default.
• W2024272267 cites W1965885099 @default.
• W2024272267 cites W1973410051 @default.
• W2024272267 cites W1977880141 @default.
• W2024272267 cites W1983105869 @default.
• W2024272267 cites W1986148031 @default.
• W2024272267 cites W2000168109 @default.
• W2024272267 cites W2000309611 @default.
• W2024272267 cites W2004330426 @default.
• W2024272267 cites W2005165257 @default.
• W2024272267 cites W2006614483 @default.
• W2024272267 cites W2009428865 @default.
• W2024272267 cites W2011615854 @default.
• W2024272267 cites W2015063854 @default.
• W2024272267 cites W2016136365 @default.
• W2024272267 cites W2016212214 @default.
• W2024272267 cites W2016752484 @default.
• W2024272267 cites W2016841341 @default.
• W2024272267 cites W2025015227 @default.
• W2024272267 cites W2025140569 @default.
• W2024272267 cites W2027529467 @default.
• W2024272267 cites W2035651268 @default.
• W2024272267 cites W2036801973 @default.
• W2024272267 cites W2047373064 @default.
• W2024272267 cites W2048808543 @default.
• W2024272267 cites W2054915008 @default.
• W2024272267 cites W2056269174 @default.
• W2024272267 cites W2056406543 @default.
• W2024272267 cites W2060598691 @default.
• W2024272267 cites W2070910260 @default.
• W2024272267 cites W2071982564 @default.
• W2024272267 cites W2073068696 @default.
• W2024272267 cites W2073660246 @default.
• W2024272267 cites W2091567823 @default.
• W2024272267 cites W2095236407 @default.
• W2024272267 cites W2098524104 @default.
• W2024272267 cites W2099685366 @default.
• W2024272267 cites W2107227663 @default.
• W2024272267 cites W2121504505 @default.
• W2024272267 cites W2129151536 @default.
• W2024272267 cites W2133403209 @default.
• W2024272267 cites W2138326915 @default.
• W2024272267 cites W2144150432 @default.
• W2024272267 cites W2146545108 @default.
• W2024272267 cites W2146783573 @default.
• W2024272267 cites W2147451073 @default.
• W2024272267 cites W2153037734 @default.
• W2024272267 cites W2161905646 @default.
• W2024272267 cites W2162317699 @default.
• W2024272267 cites W4256400035 @default.
• W2024272267 doi "https://doi.org/10.1074/jbc.m114.547976" @default.
• W2024272267 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4007474" @default.
• W2024272267 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24627493" @default.
• W2024272267 hasPublicationYear "2014" @default.
• W2024272267 type Work @default.
• W2024272267 sameAs 2024272267 @default.
• W2024272267 citedByCount "36" @default.
• W2024272267 countsByYear W20242722672015 @default.
• W2024272267 countsByYear W20242722672016 @default.
• W2024272267 countsByYear W20242722672017 @default.
• W2024272267 countsByYear W20242722672018 @default.
• W2024272267 countsByYear W20242722672020 @default.
• W2024272267 countsByYear W20242722672021 @default.
• W2024272267 countsByYear W20242722672022 @default.
• W2024272267 countsByYear W20242722672023 @default.
• W2024272267 crossrefType "journal-article" @default.
• W2024272267 hasAuthorship W2024272267A5008926411 @default.
• W2024272267 hasAuthorship W2024272267A5016966501 @default.
• W2024272267 hasAuthorship W2024272267A5026743647 @default.
• W2024272267 hasAuthorship W2024272267A5045325794 @default.
• W2024272267 hasAuthorship W2024272267A5045963974 @default.
• W2024272267 hasAuthorship W2024272267A5051529023 @default.
• W2024272267 hasAuthorship W2024272267A5076898649 @default.
• W2024272267 hasAuthorship W2024272267A5079347888 @default.
• W2024272267 hasBestOaLocation W20242722671 @default.
• W2024272267 hasConcept C104317684 @default.
• W2024272267 hasConcept C12554922 @default.
• W2024272267 hasConcept C134018914 @default.
• W2024272267 hasConcept C150194340 @default.
• W2024272267 hasConcept C158448853 @default.
• W2024272267 hasConcept C17093226 @default.
• W2024272267 hasConcept C185592680 @default.

• next