FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024276938> ?p ?o ?g. }

• W2024276938 endingPage "2343" @default.
• W2024276938 startingPage "2335" @default.
• W2024276938 abstract "Many metabolic processes in chondrocytes thought to contribute to age-related changes in the extracellular matrix are influenced by known roles of Hsp90. Age-related decreases in the level of Hsp90 have been documented in numerous cell types and could contribute to cartilage degeneration. The aim of this study was to investigate the roles of age and Hsp90 in insulin-like growth factor 1 (IGF-1) and interleukin-1beta (IL-1beta) signaling in chondrocytes.Levels of Hsp90 messenger RNA (mRNA) and protein, with respect to age, were determined by quantitative real-time polymerase chain reaction (PCR) and Western blot analysis, respectively. The Hsp90 inhibitor geldanamycin (50 nM, 100 nM, or 500 nM) was used to assess age-related responses to Hsp90 with concurrent IGF-1 or IL-1beta stimulation of chondrocytes. Quantitative real-time PCR was used to measure COL2A1 and matrix metalloproteinase 13 (MMP13) gene expression; Western blot analysis was performed to determine the phosphorylation status of p42/44 and Akt/protein kinase B.The effects of Hsp90 inhibition with geldanamycin were concentration dependent. Inhibition of Hsp90 with 100 nM or 500 nM geldanamycin blocked IGF-1-induced cell proliferation, Akt and p42/44 activation, and COL2A1 expression. Basal and IL-1beta-induced up-regulation of MMP13 mRNA was blocked by all concentrations of geldanamycin tested. Gain-of-function assays with Hsp90 resulted in increased expression of MMP13 mRNA.These results suggest that Hsp90 is involved in opposing signaling pathways of cartilage homeostasis, and that catabolic responses are more sensitive to Hsp90 inhibition than are anabolic responses. Further studies are needed to determine the role of Hsp90 inhibition in osteoarthritis in order to assess its potential as a therapeutic target." @default.
• W2024276938 created "2016-06-24" @default.
• W2024276938 creator A5000471259 @default.
• W2024276938 creator A5034735591 @default.
• W2024276938 creator A5050177676 @default.
• W2024276938 creator A5059515326 @default.
• W2024276938 date "2007-01-01" @default.
• W2024276938 modified "2023-10-07" @default.
• W2024276938 title "Hsp90 mediates insulin-like growth factor 1 and interleukin-1β signaling in an age-dependent manner in equine articular chondrocytes" @default.
• W2024276938 cites W1566112956 @default.
• W2024276938 cites W1578095196 @default.
• W2024276938 cites W1899997617 @default.
• W2024276938 cites W1907180716 @default.
• W2024276938 cites W1970672986 @default.
• W2024276938 cites W1973341497 @default.
• W2024276938 cites W1973723400 @default.
• W2024276938 cites W1976146332 @default.
• W2024276938 cites W1976619017 @default.
• W2024276938 cites W1976980728 @default.
• W2024276938 cites W1985406857 @default.
• W2024276938 cites W1990844960 @default.
• W2024276938 cites W1993902022 @default.
• W2024276938 cites W1994374256 @default.
• W2024276938 cites W1998673798 @default.
• W2024276938 cites W2000756519 @default.
• W2024276938 cites W2000929246 @default.
• W2024276938 cites W2001215449 @default.
• W2024276938 cites W2007449623 @default.
• W2024276938 cites W2016648459 @default.
• W2024276938 cites W2026479158 @default.
• W2024276938 cites W2030173545 @default.
• W2024276938 cites W2030876024 @default.
• W2024276938 cites W2036428484 @default.
• W2024276938 cites W2040196552 @default.
• W2024276938 cites W2041522326 @default.
• W2024276938 cites W2043870138 @default.
• W2024276938 cites W2053108974 @default.
• W2024276938 cites W2055012138 @default.
• W2024276938 cites W2063795634 @default.
• W2024276938 cites W2074021321 @default.
• W2024276938 cites W2078884339 @default.
• W2024276938 cites W2082390299 @default.
• W2024276938 cites W2085612955 @default.
• W2024276938 cites W2098115255 @default.
• W2024276938 cites W2100615291 @default.
• W2024276938 cites W2109142422 @default.
• W2024276938 cites W2118089705 @default.
• W2024276938 cites W2119436088 @default.
• W2024276938 cites W2131529156 @default.
• W2024276938 cites W2145283869 @default.
• W2024276938 cites W2157719302 @default.
• W2024276938 cites W2158060338 @default.
• W2024276938 cites W2159063415 @default.
• W2024276938 cites W2159315880 @default.
• W2024276938 cites W2168221175 @default.
• W2024276938 cites W2320336304 @default.
• W2024276938 doi "https://doi.org/10.1002/art.22664" @default.
• W2024276938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17599753" @default.
• W2024276938 hasPublicationYear "2007" @default.
• W2024276938 type Work @default.
• W2024276938 sameAs 2024276938 @default.
• W2024276938 citedByCount "31" @default.
• W2024276938 countsByYear W20242769382012 @default.
• W2024276938 countsByYear W20242769382013 @default.
• W2024276938 countsByYear W20242769382014 @default.
• W2024276938 countsByYear W20242769382015 @default.
• W2024276938 countsByYear W20242769382016 @default.
• W2024276938 countsByYear W20242769382017 @default.
• W2024276938 countsByYear W20242769382018 @default.
• W2024276938 countsByYear W20242769382019 @default.
• W2024276938 countsByYear W20242769382020 @default.
• W2024276938 countsByYear W20242769382023 @default.
• W2024276938 crossrefType "journal-article" @default.
• W2024276938 hasAuthorship W2024276938A5000471259 @default.
• W2024276938 hasAuthorship W2024276938A5034735591 @default.
• W2024276938 hasAuthorship W2024276938A5050177676 @default.
• W2024276938 hasAuthorship W2024276938A5059515326 @default.
• W2024276938 hasConcept C104317684 @default.
• W2024276938 hasConcept C153911025 @default.
• W2024276938 hasConcept C170493617 @default.
• W2024276938 hasConcept C185592680 @default.
• W2024276938 hasConcept C205260736 @default.
• W2024276938 hasConcept C2775932338 @default.
• W2024276938 hasConcept C2775960820 @default.
• W2024276938 hasConcept C2777560012 @default.
• W2024276938 hasConcept C2780297055 @default.
• W2024276938 hasConcept C2780689927 @default.
• W2024276938 hasConcept C55493867 @default.
• W2024276938 hasConcept C62478195 @default.
• W2024276938 hasConcept C75217442 @default.
• W2024276938 hasConcept C86803240 @default.
• W2024276938 hasConcept C95444343 @default.
• W2024276938 hasConceptScore W2024276938C104317684 @default.
• W2024276938 hasConceptScore W2024276938C153911025 @default.
• W2024276938 hasConceptScore W2024276938C170493617 @default.
• W2024276938 hasConceptScore W2024276938C185592680 @default.
• W2024276938 hasConceptScore W2024276938C205260736 @default.
• W2024276938 hasConceptScore W2024276938C2775932338 @default.

• next