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- W2024278004 abstract "The increased use of ribonucleic acid (RNA) in basic biological research and in vitro diagnostic applications has prompted the demand for large-scale, costeffective RNA production strategies. RNA is currently made by either direct chemical or enzymatic synthesis. Scale-up is prohibitive due to the high material costs coupled with the limitations of batch-mode processes. Our approach is to harness the transcriptional activity of purified bacteriophage T7 RNA polymerase using solid-phase DNA templates in a continuous-flow, stirred-cell bioreactor. A direct comparison of activity reveals that immobilized DNA templates are transcribed at about one-half the initial rate of solution phase templates in conventional batch reactions. . Despite this reduced transcription rate, equivalent yields of RNA are obtained if the reaction time period is extended. Immobilized templates store stably for periods of several months and are easily recovered from batch reactions for subsequent reuse. These findings indicate that solid-phase templates are ideal for continuous-flow and semi-batch applications, particularly since they are selectively retained in the bioreactor and thereby further facilitate downstream purification. Finally, we demonstrate this process by transcribing RNA from immobilized DNA templates in a stirred-cell reactor operated in a semi-batch mode." @default.
- W2024278004 created "2016-06-24" @default.
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- W2024278004 date "1993-01-01" @default.
- W2024278004 modified "2023-09-27" @default.
- W2024278004 title "Proceedings of the 23rd Annual Biochemical Engineering Symposium" @default.
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