FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024289375> ?p ?o ?g. }

• W2024289375 endingPage "3113" @default.
• W2024289375 startingPage "3108" @default.
• W2024289375 abstract "Apramycin (APR) residue in food of animal origin can cause harmful effects on human health. In this study, a monoclonal antibody (mAb) was successfully produced using APR–BSA as immunogen, which was prepared by using the glutaraldehyde method. mAb 2A2 showed low cross-reactivity (<0.1%) with other aminoglycoside antibiotics, and its IC50 value was 0.35 ng/mL. On the basis of this mAb, a novel immunoassay in the format of an immunoaffinity test column (IATC) was developed. An immunoaffinity column filled with anti-APR antibody–Sepharose 4B gel was used as solid phase. APR in sample and HRP–APR conjugate compete with each other for the limited antibody on the column. The approach was able to give a naked-eye color signal for the detection of analyte. A blue color appears for negative results and no color for positive. The method was then successfully applied to the detection of APR in animal-origin food. To further evaluate the assay, direct competitive ELISA (dcELISA) based on the same antibody was developed for comparison in different aspects. Compared to the dcELISA, the detection time of IATC is shortened to 20 min, whereas a similar sensitivity for various samples was observed. The limits of detection (LOD) for raw milk, muscles, and livers are 3 ng/mL, 3 μg/kg, and 10 μg/kg, respectively." @default.
• W2024289375 created "2016-06-24" @default.
• W2024289375 creator A5006753194 @default.
• W2024289375 creator A5008181998 @default.
• W2024289375 creator A5008677770 @default.
• W2024289375 creator A5038238437 @default.
• W2024289375 creator A5056228950 @default.
• W2024289375 creator A5057496704 @default.
• W2024289375 creator A5079478620 @default.
• W2024289375 date "2014-03-31" @default.
• W2024289375 modified "2023-09-22" @default.
• W2024289375 title "Production of Monoclonal Antibody and Development of a New Immunoassay for Apramycin in Food" @default.
• W2024289375 cites W1963776010 @default.
• W2024289375 cites W1976929957 @default.
• W2024289375 cites W1986506548 @default.
• W2024289375 cites W1987109588 @default.
• W2024289375 cites W1996999431 @default.
• W2024289375 cites W2003803780 @default.
• W2024289375 cites W2033990005 @default.
• W2024289375 cites W2049569552 @default.
• W2024289375 cites W2070834894 @default.
• W2024289375 cites W2100595325 @default.
• W2024289375 cites W2134997780 @default.
• W2024289375 cites W2137254904 @default.
• W2024289375 cites W2159418853 @default.
• W2024289375 cites W2333801528 @default.
• W2024289375 cites W1981639855 @default.
• W2024289375 doi "https://doi.org/10.1021/jf405379r" @default.
• W2024289375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24661253" @default.
• W2024289375 hasPublicationYear "2014" @default.
• W2024289375 type Work @default.
• W2024289375 sameAs 2024289375 @default.
• W2024289375 citedByCount "25" @default.
• W2024289375 countsByYear W20242893752014 @default.
• W2024289375 countsByYear W20242893752015 @default.
• W2024289375 countsByYear W20242893752016 @default.
• W2024289375 countsByYear W20242893752017 @default.
• W2024289375 countsByYear W20242893752018 @default.
• W2024289375 countsByYear W20242893752019 @default.
• W2024289375 countsByYear W20242893752020 @default.
• W2024289375 countsByYear W20242893752022 @default.
• W2024289375 countsByYear W20242893752023 @default.
• W2024289375 crossrefType "journal-article" @default.
• W2024289375 hasAuthorship W2024289375A5006753194 @default.
• W2024289375 hasAuthorship W2024289375A5008181998 @default.
• W2024289375 hasAuthorship W2024289375A5008677770 @default.
• W2024289375 hasAuthorship W2024289375A5038238437 @default.
• W2024289375 hasAuthorship W2024289375A5056228950 @default.
• W2024289375 hasAuthorship W2024289375A5057496704 @default.
• W2024289375 hasAuthorship W2024289375A5079478620 @default.
• W2024289375 hasConcept C10390740 @default.
• W2024289375 hasConcept C119128265 @default.
• W2024289375 hasConcept C134306372 @default.
• W2024289375 hasConcept C142724271 @default.
• W2024289375 hasConcept C153911025 @default.
• W2024289375 hasConcept C159654299 @default.
• W2024289375 hasConcept C185592680 @default.
• W2024289375 hasConcept C197336794 @default.
• W2024289375 hasConcept C203014093 @default.
• W2024289375 hasConcept C204628709 @default.
• W2024289375 hasConcept C204787440 @default.
• W2024289375 hasConcept C2779142602 @default.
• W2024289375 hasConcept C2779909307 @default.
• W2024289375 hasConcept C2780367336 @default.
• W2024289375 hasConcept C2780420688 @default.
• W2024289375 hasConcept C3020064264 @default.
• W2024289375 hasConcept C33923547 @default.
• W2024289375 hasConcept C43617362 @default.
• W2024289375 hasConcept C542903549 @default.
• W2024289375 hasConcept C55493867 @default.
• W2024289375 hasConcept C71924100 @default.
• W2024289375 hasConcept C86803240 @default.
• W2024289375 hasConceptScore W2024289375C10390740 @default.
• W2024289375 hasConceptScore W2024289375C119128265 @default.
• W2024289375 hasConceptScore W2024289375C134306372 @default.
• W2024289375 hasConceptScore W2024289375C142724271 @default.
• W2024289375 hasConceptScore W2024289375C153911025 @default.
• W2024289375 hasConceptScore W2024289375C159654299 @default.
• W2024289375 hasConceptScore W2024289375C185592680 @default.
• W2024289375 hasConceptScore W2024289375C197336794 @default.
• W2024289375 hasConceptScore W2024289375C203014093 @default.
• W2024289375 hasConceptScore W2024289375C204628709 @default.
• W2024289375 hasConceptScore W2024289375C204787440 @default.
• W2024289375 hasConceptScore W2024289375C2779142602 @default.
• W2024289375 hasConceptScore W2024289375C2779909307 @default.
• W2024289375 hasConceptScore W2024289375C2780367336 @default.
• W2024289375 hasConceptScore W2024289375C2780420688 @default.
• W2024289375 hasConceptScore W2024289375C3020064264 @default.
• W2024289375 hasConceptScore W2024289375C33923547 @default.
• W2024289375 hasConceptScore W2024289375C43617362 @default.
• W2024289375 hasConceptScore W2024289375C542903549 @default.
• W2024289375 hasConceptScore W2024289375C55493867 @default.
• W2024289375 hasConceptScore W2024289375C71924100 @default.
• W2024289375 hasConceptScore W2024289375C86803240 @default.
• W2024289375 hasIssue "14" @default.
• W2024289375 hasLocation W20242893751 @default.
• W2024289375 hasLocation W20242893752 @default.
• W2024289375 hasOpenAccess W2024289375 @default.

• next