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- W2024295146 abstract "Abstract The solution behavior of the single‐stranded CCAAT‐containing octamer 1 , d(AGCCAATA), that comprises part of the nuclear factor I (NF‐I) recognition site at the origin of replication of human adenovirus has been studied by nmr spectroscopy at 500 and 600 MHz. Proton resonance assignments for 1 were aided by selective 13 C enrichment at C1′ of A 1 or A 5 . High‐resolution 13 C‐ 1 H heteronuclear multiple‐bond coherence spectra of the 13 C‐labeled oligomers permitted the selective detection of furanosyl ring protons within each labeled residue due to short‐ and long‐range 13 C‐ 1 H couplings to the enriched C1′. The resulting assignments provided firm starting points in the interpretation of double quantum filtered correlated spectra, yielding information supplemented by total correlated spectroscopy (TOCSY) and rotating frame nuclear Overhauser effect spectroscopic data to completely assign the 1 H‐nmr spectrum of 1 and extract 3 J HH values for furanose con‐formational analysis. Several 13 C‐ 1 H spin‐coupling constants within the 13 C‐enriched A 1 or A 5 residues were measured from cross‐peak shifts in TOCSY spectra, and their signs determined by inspection of the relative orientations of these shifts. 1 H‐ 2 ‐H and 13 C‐ 1 H spin‐couplings both indicate a preference (> 75%) for south (C2′‐ endo ) conformations by the furanosyl rings of 1 . © 1994 John Wiley & Sons, Inc." @default.
- W2024295146 created "2016-06-24" @default.
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- W2024295146 date "1994-09-01" @default.
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- W2024295146 title "13C-labeled oligodeoxyribonucleotides: A solution study of a CCAAT-containing sequence at the nuclear factor I recognition site of human adenovirus" @default.
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- W2024295146 doi "https://doi.org/10.1002/bip.360340907" @default.
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