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• W2024301716 abstract "Phogrin (IA2-β) is an integral membrane protein of dense-core vesicles in neuroendocrine cells. We have examined the recycling of endogenous phogrin following exocytosis in insulin secreting Min6 β-cells by monitoring stimulus dependent-uptake of antibodies directed against the lumenal domain of the protein. While low levels of internalized phogrin accumulated in LAMP1-positive lysosomes, more than 35% of internalized phogrin recycled back to an insulin-positive compartment and could return to the cell surface during a second exocytic stimulation. The recycling phogrin transited a syntaxin 6-positive compartment but did not appear to go through the TGN38-positive trans Golgi network. The results suggest a model in which secretory membrane components can recycle from the endosomal system to immature secretory granules without interaction with the major portion of the TGN." @default.
• W2024301716 created "2016-06-24" @default.
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• W2024301716 date "2004-11-01" @default.
• W2024301716 modified "2023-09-27" @default.
• W2024301716 title "Recycling of the dense-core vesicle membrane protein phogrin in Min6 β-cells" @default.
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• W2024301716 doi "https://doi.org/10.1016/j.bbrc.2004.09.147" @default.
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