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• W2024332593 abstract "Propionibacterium freudenreichii subsp. shermanii naturally forms propionic acid as the main fermentation product with acetate and succinate as two major by-products. In this study, overexpressing the native propionyl-CoA:succinate CoA transferase (CoAT) in P. shermanii was investigated to evaluate its effects on propionic acid fermentation with glucose, glycerol, and their mixtures as carbon source. In general, the mutant produced more propionic acid, with up to 10% increase in yield (0.62 vs. 0.56 g/g) and 46% increase in productivity (0.41 vs. 0.28 g/L h), depending on the fermentation conditions. The mutant also produced less acetate and succinate, with the ratios of propionate to acetate (P/A) and succinate (P/S) in the final product increased 50% and 23%, respectively, in the co-fermentation of glucose/glycerol. Metabolic flux analysis elucidated that CoAT overexpression diverted more carbon fluxes toward propionic acid, resulting in higher propionic acid purity and a preference for glycerol over glucose as carbon source." @default.
• W2024332593 created "2016-06-24" @default.
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• W2024332593 date "2015-01-01" @default.
• W2024332593 modified "2023-10-16" @default.
• W2024332593 title "Engineering Propionibacterium freudenreichii subsp. shermanii for enhanced propionic acid fermentation: Effects of overexpressing propionyl-CoA:Succinate CoA transferase" @default.
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• W2024332593 doi "https://doi.org/10.1016/j.ymben.2014.10.005" @default.
• W2024332593 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25447642" @default.
• W2024332593 hasPublicationYear "2015" @default.
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