Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024334150> ?p ?o ?g. }
- W2024334150 endingPage "7131" @default.
- W2024334150 startingPage "7123" @default.
- W2024334150 abstract "Pro-survival members of the Bcl-2 family of proteins restrain the pro-apoptotic activity of Bax, either directly through interactions with Bax or indirectly by sequestration of activator BH3-only proteins, or both. Mutations in Bax that promote apoptosis can provide insight into how Bax is regulated. Here, we describe crystal structures of the pro-survival proteins Mcl-1 and Bcl-x(L) in complex with a 34-mer peptide from Bax that encompasses its BH3 domain. These structures reveal canonical interactions between four signature hydrophobic amino acids from the BaxBH3 domain and the BH3-binding groove of the pro-survival proteins. In both structures, Met-74 from the Bax peptide engages with the BH3-binding groove in a fifth hydrophobic interaction. Various Bax Met-74 mutants disrupt interactions between Bax and all pro-survival proteins, but these Bax mutants retain pro-apoptotic activity. Bax/Bak-deficient mouse embryonic fibroblast cells reconstituted with several Bax Met-74 mutants are more sensitive to the BH3 mimetic compound ABT-737 as compared with cells expressing wild-type Bax. Furthermore, the cells expressing Bax Met-74 mutants are less viable in colony assays even in the absence of an external apoptotic stimulus. These results support a model in which direct restraint of Bax by pro-survival Bcl-2 proteins is a barrier to apoptosis." @default.
- W2024334150 created "2016-06-24" @default.
- W2024334150 creator A5038439002 @default.
- W2024334150 creator A5051473344 @default.
- W2024334150 creator A5063424737 @default.
- W2024334150 creator A5075108697 @default.
- W2024334150 creator A5085303184 @default.
- W2024334150 creator A5091684098 @default.
- W2024334150 date "2011-03-01" @default.
- W2024334150 modified "2023-10-17" @default.
- W2024334150 title "Mutation to Bax beyond the BH3 Domain Disrupts Interactions with Pro-survival Proteins and Promotes Apoptosis" @default.
- W2024334150 cites W1539796472 @default.
- W2024334150 cites W1825915710 @default.
- W2024334150 cites W1835922068 @default.
- W2024334150 cites W1965071591 @default.
- W2024334150 cites W1965217295 @default.
- W2024334150 cites W1977258127 @default.
- W2024334150 cites W1983149947 @default.
- W2024334150 cites W1984547206 @default.
- W2024334150 cites W1984636849 @default.
- W2024334150 cites W1990458209 @default.
- W2024334150 cites W1994286350 @default.
- W2024334150 cites W2000093730 @default.
- W2024334150 cites W2002919668 @default.
- W2024334150 cites W2006941663 @default.
- W2024334150 cites W2008376982 @default.
- W2024334150 cites W2008841394 @default.
- W2024334150 cites W2011306037 @default.
- W2024334150 cites W2012076962 @default.
- W2024334150 cites W2014985938 @default.
- W2024334150 cites W2017991274 @default.
- W2024334150 cites W2018104469 @default.
- W2024334150 cites W2024255120 @default.
- W2024334150 cites W2026873116 @default.
- W2024334150 cites W2027395744 @default.
- W2024334150 cites W2032366675 @default.
- W2024334150 cites W2033013637 @default.
- W2024334150 cites W2038840577 @default.
- W2024334150 cites W2049172807 @default.
- W2024334150 cites W2058279047 @default.
- W2024334150 cites W2062555640 @default.
- W2024334150 cites W2072201965 @default.
- W2024334150 cites W2073028327 @default.
- W2024334150 cites W2073571990 @default.
- W2024334150 cites W2082055490 @default.
- W2024334150 cites W2082293892 @default.
- W2024334150 cites W2085419794 @default.
- W2024334150 cites W2085870553 @default.
- W2024334150 cites W2089082314 @default.
- W2024334150 cites W2091483784 @default.
- W2024334150 cites W2094052642 @default.
- W2024334150 cites W2097007474 @default.
- W2024334150 cites W2100762532 @default.
- W2024334150 cites W2106799894 @default.
- W2024334150 cites W2109184569 @default.
- W2024334150 cites W2114001234 @default.
- W2024334150 cites W2116177527 @default.
- W2024334150 cites W2134979684 @default.
- W2024334150 cites W2144081223 @default.
- W2024334150 cites W2147318282 @default.
- W2024334150 cites W2150315067 @default.
- W2024334150 cites W2150687797 @default.
- W2024334150 cites W2162445214 @default.
- W2024334150 cites W2162560719 @default.
- W2024334150 cites W2165208382 @default.
- W2024334150 cites W2166467949 @default.
- W2024334150 cites W80462140 @default.
- W2024334150 doi "https://doi.org/10.1074/jbc.m110.161281" @default.
- W2024334150 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3044969" @default.
- W2024334150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21199865" @default.
- W2024334150 hasPublicationYear "2011" @default.
- W2024334150 type Work @default.
- W2024334150 sameAs 2024334150 @default.
- W2024334150 citedByCount "97" @default.
- W2024334150 countsByYear W20243341502012 @default.
- W2024334150 countsByYear W20243341502013 @default.
- W2024334150 countsByYear W20243341502014 @default.
- W2024334150 countsByYear W20243341502015 @default.
- W2024334150 countsByYear W20243341502016 @default.
- W2024334150 countsByYear W20243341502017 @default.
- W2024334150 countsByYear W20243341502018 @default.
- W2024334150 countsByYear W20243341502019 @default.
- W2024334150 countsByYear W20243341502020 @default.
- W2024334150 countsByYear W20243341502021 @default.
- W2024334150 countsByYear W20243341502022 @default.
- W2024334150 countsByYear W20243341502023 @default.
- W2024334150 crossrefType "journal-article" @default.
- W2024334150 hasAuthorship W2024334150A5038439002 @default.
- W2024334150 hasAuthorship W2024334150A5051473344 @default.
- W2024334150 hasAuthorship W2024334150A5063424737 @default.
- W2024334150 hasAuthorship W2024334150A5075108697 @default.
- W2024334150 hasAuthorship W2024334150A5085303184 @default.
- W2024334150 hasAuthorship W2024334150A5091684098 @default.
- W2024334150 hasBestOaLocation W20243341501 @default.
- W2024334150 hasConcept C104317684 @default.
- W2024334150 hasConcept C12519072 @default.
- W2024334150 hasConcept C143065580 @default.
- W2024334150 hasConcept C149150037 @default.