Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024334977> ?p ?o ?g. }
- W2024334977 endingPage "29" @default.
- W2024334977 startingPage "18" @default.
- W2024334977 abstract "The potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell therapy has just entered a new era. Here, we report seven hESC lines (SEES-1, -2, -3, -4, -5, -6, and -7). Four of them were derived and maintained on irradiated human mesenchymal stem cells (hMSCs) grown in xenogeneic-free defined media and substrate. Xenogeneic-free hMSCs isolated from the subcutaneous tissue of extra fingers from individuals with polydactyly showed appropriate potentials as feeder layers in the pluripotency and growth of hESCs. In this report, we describe a comprehensive characterization of these newly derived SEES cell lines. In addition, we developed a scalable culture system for hESCs having high biological safety by using gamma-irradiated serum replacement and pharmaceutical-grade recombinant basic fibroblast growth factor (bFGF, also known as trafermin). This is first report describing the maintenance of hESC pluripotency using pharmaceutical-grade human recombinant bFGF (trafermin) and gamma-irradiated serum replacement. Our defined medium system provides a path to scalability in Good Manufacturing Practice (GMP) settings for the generation of clinically relevant cell types from pluripotent cells for therapeutic applications." @default.
- W2024334977 created "2016-06-24" @default.
- W2024334977 creator A5004608361 @default.
- W2024334977 creator A5008376932 @default.
- W2024334977 creator A5016298609 @default.
- W2024334977 creator A5027421629 @default.
- W2024334977 creator A5028009766 @default.
- W2024334977 creator A5054735695 @default.
- W2024334977 creator A5059970716 @default.
- W2024334977 creator A5061116323 @default.
- W2024334977 creator A5063243033 @default.
- W2024334977 creator A5068456830 @default.
- W2024334977 creator A5072022590 @default.
- W2024334977 creator A5085938926 @default.
- W2024334977 date "2015-06-01" @default.
- W2024334977 modified "2023-10-12" @default.
- W2024334977 title "Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells" @default.
- W2024334977 cites W1599568627 @default.
- W2024334977 cites W1667282266 @default.
- W2024334977 cites W1972564906 @default.
- W2024334977 cites W1976628761 @default.
- W2024334977 cites W1986670988 @default.
- W2024334977 cites W1988865085 @default.
- W2024334977 cites W2001499488 @default.
- W2024334977 cites W2003559649 @default.
- W2024334977 cites W2025875013 @default.
- W2024334977 cites W2035586023 @default.
- W2024334977 cites W2037388456 @default.
- W2024334977 cites W2038723730 @default.
- W2024334977 cites W2040914220 @default.
- W2024334977 cites W2045048112 @default.
- W2024334977 cites W2047172312 @default.
- W2024334977 cites W2048706786 @default.
- W2024334977 cites W2053436608 @default.
- W2024334977 cites W2059186085 @default.
- W2024334977 cites W2072625559 @default.
- W2024334977 cites W2086943348 @default.
- W2024334977 cites W2091790925 @default.
- W2024334977 cites W2093813223 @default.
- W2024334977 cites W2106657978 @default.
- W2024334977 cites W2108285674 @default.
- W2024334977 cites W2110649539 @default.
- W2024334977 cites W2120121992 @default.
- W2024334977 cites W2131524695 @default.
- W2024334977 cites W2133501984 @default.
- W2024334977 cites W2149838334 @default.
- W2024334977 cites W2155862946 @default.
- W2024334977 cites W2161376566 @default.
- W2024334977 cites W4247033596 @default.
- W2024334977 doi "https://doi.org/10.1016/j.reth.2014.12.004" @default.
- W2024334977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6581821" @default.
- W2024334977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31245438" @default.
- W2024334977 hasPublicationYear "2015" @default.
- W2024334977 type Work @default.
- W2024334977 sameAs 2024334977 @default.
- W2024334977 citedByCount "38" @default.
- W2024334977 countsByYear W20243349772015 @default.
- W2024334977 countsByYear W20243349772016 @default.
- W2024334977 countsByYear W20243349772017 @default.
- W2024334977 countsByYear W20243349772018 @default.
- W2024334977 countsByYear W20243349772019 @default.
- W2024334977 countsByYear W20243349772020 @default.
- W2024334977 countsByYear W20243349772021 @default.
- W2024334977 countsByYear W20243349772022 @default.
- W2024334977 countsByYear W20243349772023 @default.
- W2024334977 crossrefType "journal-article" @default.
- W2024334977 hasAuthorship W2024334977A5004608361 @default.
- W2024334977 hasAuthorship W2024334977A5008376932 @default.
- W2024334977 hasAuthorship W2024334977A5016298609 @default.
- W2024334977 hasAuthorship W2024334977A5027421629 @default.
- W2024334977 hasAuthorship W2024334977A5028009766 @default.
- W2024334977 hasAuthorship W2024334977A5054735695 @default.
- W2024334977 hasAuthorship W2024334977A5059970716 @default.
- W2024334977 hasAuthorship W2024334977A5061116323 @default.
- W2024334977 hasAuthorship W2024334977A5063243033 @default.
- W2024334977 hasAuthorship W2024334977A5068456830 @default.
- W2024334977 hasAuthorship W2024334977A5072022590 @default.
- W2024334977 hasAuthorship W2024334977A5085938926 @default.
- W2024334977 hasBestOaLocation W20243349771 @default.
- W2024334977 hasConcept C104317684 @default.
- W2024334977 hasConcept C107459253 @default.
- W2024334977 hasConcept C10854531 @default.
- W2024334977 hasConcept C136229726 @default.
- W2024334977 hasConcept C145103041 @default.
- W2024334977 hasConcept C170493617 @default.
- W2024334977 hasConcept C198826908 @default.
- W2024334977 hasConcept C203014093 @default.
- W2024334977 hasConcept C2775960820 @default.
- W2024334977 hasConcept C2777587049 @default.
- W2024334977 hasConcept C28328180 @default.
- W2024334977 hasConcept C49892992 @default.
- W2024334977 hasConcept C55493867 @default.
- W2024334977 hasConcept C71924100 @default.
- W2024334977 hasConcept C86803240 @default.
- W2024334977 hasConcept C95444343 @default.
- W2024334977 hasConceptScore W2024334977C104317684 @default.
- W2024334977 hasConceptScore W2024334977C107459253 @default.
- W2024334977 hasConceptScore W2024334977C10854531 @default.