FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024338032> ?p ?o ?g. }

• W2024338032 abstract "You have accessJournal of UrologyInfertility: Physiology, Pathophysiology, Basic Research1 Apr 20101878 DDX4 - HIGHLY SENSITIVE TESTICULAR GERM CELL MARKER AND DIAGNOSIS OF SERTOLI CELL ONLY SYNDROME Laurent Vaucher, Ronnie Fine, Anna Mielnik, Peter Schlegel, and Darius Paduch Laurent VaucherLaurent Vaucher Lausanne, Switzerland More articles by this author , Ronnie FineRonnie Fine New York, NY More articles by this author , Anna MielnikAnna Mielnik New York, NY More articles by this author , Peter SchlegelPeter Schlegel New York, NY More articles by this author , and Darius PaduchDarius Paduch New York, NY More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1831AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Sertoli cell only syndrome (SCO) on testicular biopsy, by definition, indicates lack of germ cells and as such a diagnosis theoretically makes microsurgical testicular sperm extraction contraindicated. Thus accurate diagnosis of SCO is critical in management of men with severe forms of infertility. However due to the heterogeneous architecture of human testis, assessment of testicular biopsies is often unreliable, and sperm can be found in 20% of patient inaccurately diagnosed as SCO by clinical pathologists thus one in five men would be denied TESE. The main difficulty is the identification of severely underrepresented germ cells in testicular biopsies using current accepted immunohistochemistry markers. We have already shown that DDX4 is a highly sensitive and specific marker for germ cells in ejaculate and normal human testis. In current report, we focused on clinical utility of measuring DDX4 relative expression in men with severe forms of infertility who underwent TESE. METHODS Random testicular biopsies from 35 patients were obtained through microsurgical biopsy and snap frozen in liquid nitrogen. H&E slides were analyzed using morphometric classification by academic senior pathologist. Total RNA was extracted from the small testis sample (less than 50mg), and the cDNA was used for qRTPCR using exon-intron spanning multicolor hydrolysis primers designed in our laboratory and normalized to b-Actin to measure expression of DDX4. qRT-PCR were analyzed blindly using 2nd derivative relative quantification automatic method. RESULTS Men with SCO and no sperm identified by embryologist had no expression of DDX4 and normal expression of housekeeping genes. DDX4 was expressed when sperm was identified by embryologist in the operating room despite SCO described by pathologist, and histopathology diagnosis was corrected in 8 out of 18 patients diagnosed as SCO. There were statistically significant difference in DDX4 expression between normal testis, early MA, and late MA (3.5,0.9, and 0.6 respectively, p = 0.002). CONCLUSIONS Quantitative RTPCR analysis of DDX4 expression is highly sensitive and can reliably confirm the presence of germ cells thus excluding diagnosis of SCO. This technique can accurately confirm SCO diagnosis during first biopsy and avoid unnecessary procedures to these patients who can consider adoption or donor insemination. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e730 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Laurent Vaucher Lausanne, Switzerland More articles by this author Ronnie Fine New York, NY More articles by this author Anna Mielnik New York, NY More articles by this author Peter Schlegel New York, NY More articles by this author Darius Paduch New York, NY More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ..." @default.
• W2024338032 created "2016-06-24" @default.
• W2024338032 creator A5035677010 @default.
• W2024338032 creator A5059627181 @default.
• W2024338032 creator A5062048430 @default.
• W2024338032 creator A5067242842 @default.
• W2024338032 creator A5073428580 @default.
• W2024338032 date "2010-04-01" @default.
• W2024338032 modified "2023-09-27" @default.
• W2024338032 title "1878 DDX4 - HIGHLY SENSITIVE TESTICULAR GERM CELL MARKER AND DIAGNOSIS OF SERTOLI CELL ONLY SYNDROME" @default.
• W2024338032 doi "https://doi.org/10.1016/j.juro.2010.02.1831" @default.
• W2024338032 hasPublicationYear "2010" @default.
• W2024338032 type Work @default.
• W2024338032 sameAs 2024338032 @default.
• W2024338032 citedByCount "0" @default.
• W2024338032 crossrefType "journal-article" @default.
• W2024338032 hasAuthorship W2024338032A5035677010 @default.
• W2024338032 hasAuthorship W2024338032A5059627181 @default.
• W2024338032 hasAuthorship W2024338032A5062048430 @default.
• W2024338032 hasAuthorship W2024338032A5067242842 @default.
• W2024338032 hasAuthorship W2024338032A5073428580 @default.
• W2024338032 hasConcept C104317684 @default.
• W2024338032 hasConcept C123765429 @default.
• W2024338032 hasConcept C142724271 @default.
• W2024338032 hasConcept C16685009 @default.
• W2024338032 hasConcept C2777338322 @default.
• W2024338032 hasConcept C2777688143 @default.
• W2024338032 hasConcept C2779122487 @default.
• W2024338032 hasConcept C2779234561 @default.
• W2024338032 hasConcept C2781068068 @default.
• W2024338032 hasConcept C2781087480 @default.
• W2024338032 hasConcept C29456083 @default.
• W2024338032 hasConcept C54355233 @default.
• W2024338032 hasConcept C71924100 @default.
• W2024338032 hasConcept C86803240 @default.
• W2024338032 hasConceptScore W2024338032C104317684 @default.
• W2024338032 hasConceptScore W2024338032C123765429 @default.
• W2024338032 hasConceptScore W2024338032C142724271 @default.
• W2024338032 hasConceptScore W2024338032C16685009 @default.
• W2024338032 hasConceptScore W2024338032C2777338322 @default.
• W2024338032 hasConceptScore W2024338032C2777688143 @default.
• W2024338032 hasConceptScore W2024338032C2779122487 @default.
• W2024338032 hasConceptScore W2024338032C2779234561 @default.
• W2024338032 hasConceptScore W2024338032C2781068068 @default.
• W2024338032 hasConceptScore W2024338032C2781087480 @default.
• W2024338032 hasConceptScore W2024338032C29456083 @default.
• W2024338032 hasConceptScore W2024338032C54355233 @default.
• W2024338032 hasConceptScore W2024338032C71924100 @default.
• W2024338032 hasConceptScore W2024338032C86803240 @default.
• W2024338032 hasIssue "4S" @default.
• W2024338032 hasLocation W20243380321 @default.
• W2024338032 hasOpenAccess W2024338032 @default.
• W2024338032 hasPrimaryLocation W20243380321 @default.
• W2024338032 hasRelatedWork W2067897388 @default.
• W2024338032 hasRelatedWork W2075880702 @default.
• W2024338032 hasRelatedWork W2110483617 @default.
• W2024338032 hasRelatedWork W2314375973 @default.
• W2024338032 hasRelatedWork W2729223853 @default.
• W2024338032 hasRelatedWork W2912577818 @default.
• W2024338032 hasRelatedWork W3029586861 @default.
• W2024338032 hasRelatedWork W3043398618 @default.
• W2024338032 hasRelatedWork W3131955859 @default.
• W2024338032 hasRelatedWork W4236477028 @default.
• W2024338032 hasVolume "183" @default.
• W2024338032 isParatext "false" @default.
• W2024338032 isRetracted "false" @default.
• W2024338032 magId "2024338032" @default.
• W2024338032 workType "article" @default.