FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024355926> ?p ?o ?g. }

• W2024355926 endingPage "433" @default.
• W2024355926 startingPage "427" @default.
• W2024355926 abstract "Cationic liposomes bound to plasmid DNA are currently used for in vitro and in vivo gene therapy applications, but such complexes readily form large, heterogeneous aggregates that are not appropriate for pharmaceutical development. More importantly, size heterogeneity makes studies focused on optimizing gene transfer to cells difficult to conduct or understand. For this reason we have evaluated the effect of microprobe sonication on these complexes in an effort to acheive process-controlled size homogeneity. Complexes were prepared using a 7.2 kb reporter plasmid and the following liposomal lipid combinations: DDAB/DOPE (50:50 mol%), DDAB/DOPE/PEG-PE (50:45:5 mol%), DDAB/EPC (50:50 mol%), DDAB/EPC/PEG-PE (50:45:5, 50:40:10, 50:35:15 mol%), DODAC/DOPE (50:50 mol%), and DODAC/EPC (50:50 mol%) (DDAB, dimethyldioctadecylammonium bromide; DOPE, dioleoylphos-phatidylethanolamine; PEG-PE, monomethoxypolyethylene glycol2000 succinate–distearoylphosphatidylethanolamine; EPC, egg phosphatidylcholine; DODAC, dioleoyldimethylammonium chloride). The influence of complex composition and lipid:DNA ratio was evaluated. Particle size was determined before and after complexation and again after sonication using the quasi-elastic light scattering technique. DNA integrity was assessed via agarose gel electrophoresis. Finally, gene transfection was evaluated using CHO cells that were transfected in vitro with sonicated and unsonicated complexes. It is established in this study that size reduction can occur, but this is dependent on cationic and neutral lipid composition and, in some cases, lipid:DNA ratio. Surprisingly, the process of sonication leaves a significant percentage of the plasmid DNA intact and capable of in vitro transfection. This study shows that plasmid DNA can be protected from damage due to sonication by liposome complex formation. This may indicate that more common pharmaceutical methods for size reduction which subject particles to mechanical stress may be applicable in preparation of liposome/DNA formulations for in vivo application." @default.
• W2024355926 created "2016-06-24" @default.
• W2024355926 creator A5016253178 @default.
• W2024355926 creator A5070792731 @default.
• W2024355926 creator A5073912271 @default.
• W2024355926 date "1996-04-01" @default.
• W2024355926 modified "2023-10-18" @default.
• W2024355926 title "Plasmid DNA is Protected against Ultrasonic Cavitation-Induced Damage when Complexed to Cationic Liposomes" @default.
• W2024355926 cites W1518242971 @default.
• W2024355926 cites W1554786089 @default.
• W2024355926 cites W1579604458 @default.
• W2024355926 cites W1769495290 @default.
• W2024355926 cites W1965372133 @default.
• W2024355926 cites W1969147493 @default.
• W2024355926 cites W1971290197 @default.
• W2024355926 cites W1974673092 @default.
• W2024355926 cites W1975102940 @default.
• W2024355926 cites W1982966474 @default.
• W2024355926 cites W1984718218 @default.
• W2024355926 cites W1985951463 @default.
• W2024355926 cites W1999373563 @default.
• W2024355926 cites W1999568195 @default.
• W2024355926 cites W1999598401 @default.
• W2024355926 cites W2001577610 @default.
• W2024355926 cites W2009038500 @default.
• W2024355926 cites W2009482334 @default.
• W2024355926 cites W2022719485 @default.
• W2024355926 cites W2027696154 @default.
• W2024355926 cites W2034642422 @default.
• W2024355926 cites W2036839119 @default.
• W2024355926 cites W2043954711 @default.
• W2024355926 cites W2056584136 @default.
• W2024355926 cites W2058383496 @default.
• W2024355926 cites W2062412252 @default.
• W2024355926 cites W2063250637 @default.
• W2024355926 cites W2064100334 @default.
• W2024355926 cites W2064733212 @default.
• W2024355926 cites W2064844179 @default.
• W2024355926 cites W2065510348 @default.
• W2024355926 cites W2067892292 @default.
• W2024355926 cites W2079558579 @default.
• W2024355926 cites W2086836411 @default.
• W2024355926 cites W2087924735 @default.
• W2024355926 cites W2101918969 @default.
• W2024355926 cites W2109545140 @default.
• W2024355926 cites W2126662679 @default.
• W2024355926 cites W2152311269 @default.
• W2024355926 cites W2160025282 @default.
• W2024355926 cites W2167164908 @default.
• W2024355926 cites W2168146715 @default.
• W2024355926 cites W2168776854 @default.
• W2024355926 cites W2171159014 @default.
• W2024355926 cites W2199184069 @default.
• W2024355926 cites W22185118 @default.
• W2024355926 cites W2240466886 @default.
• W2024355926 cites W2316581684 @default.
• W2024355926 cites W4250570532 @default.
• W2024355926 doi "https://doi.org/10.1021/js9504752" @default.
• W2024355926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8901083" @default.
• W2024355926 hasPublicationYear "1996" @default.
• W2024355926 type Work @default.
• W2024355926 sameAs 2024355926 @default.
• W2024355926 citedByCount "68" @default.
• W2024355926 countsByYear W20243559262012 @default.
• W2024355926 countsByYear W20243559262013 @default.
• W2024355926 countsByYear W20243559262014 @default.
• W2024355926 countsByYear W20243559262016 @default.
• W2024355926 countsByYear W20243559262018 @default.
• W2024355926 countsByYear W20243559262019 @default.
• W2024355926 countsByYear W20243559262020 @default.
• W2024355926 crossrefType "journal-article" @default.
• W2024355926 hasAuthorship W2024355926A5016253178 @default.
• W2024355926 hasAuthorship W2024355926A5070792731 @default.
• W2024355926 hasAuthorship W2024355926A5073912271 @default.
• W2024355926 hasConcept C10138342 @default.
• W2024355926 hasConcept C104317684 @default.
• W2024355926 hasConcept C104628117 @default.
• W2024355926 hasConcept C116084860 @default.
• W2024355926 hasConcept C135983454 @default.
• W2024355926 hasConcept C155887181 @default.
• W2024355926 hasConcept C162324750 @default.
• W2024355926 hasConcept C178790620 @default.
• W2024355926 hasConcept C183882617 @default.
• W2024355926 hasConcept C185154212 @default.
• W2024355926 hasConcept C185592680 @default.
• W2024355926 hasConcept C202751555 @default.
• W2024355926 hasConcept C22744801 @default.
• W2024355926 hasConcept C2776330855 @default.
• W2024355926 hasConcept C2776907736 @default.
• W2024355926 hasConcept C2778271344 @default.
• W2024355926 hasConcept C2778476535 @default.
• W2024355926 hasConcept C2778918659 @default.
• W2024355926 hasConcept C41625074 @default.
• W2024355926 hasConcept C43617362 @default.
• W2024355926 hasConcept C54009773 @default.
• W2024355926 hasConcept C54400483 @default.
• W2024355926 hasConcept C552990157 @default.
• W2024355926 hasConcept C55493867 @default.

• next