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• W2024362885 abstract "An attempt has been made to evaluate some of the kinetic parameters governing the uptake of amino acids by muscle by measuring the steady-state concentration ratios established on incubation of the tissue in vitro. The data are analysed in terms of the steady state resulting from a balance between saturable uptake and efflux processes in conjuction with diffusion. For aminoisobutyrate and cycloleucine the data were compatible with efflux being solely by diffusion, but it was also possible to fit the data to conditions such that efflux by a saturable process and by diffusion were both occurring. Measurement of initial entry rates after preloading hearts with aminoisobutyrate gave results consistent with the first possibility only. Such measurements also suggested that the parameters governing a saturable uptake process changed during the course of uptake, but that the rate constant for diffusion remained the same. The rate of approach to equilibrium was found to be faster the higher the external concentration of amino acid. Both these observations would be consistent with accumulated amino acid progressively interfering with further operation of a carrier. For aminoisobutyrate the enhancement of accumulation in the presence of insulin appeared to result from an increase in the maximal velocity of the transport rate rather than from any change in apparent Km. For cycloleucine there was some diminution in the Km with insulin but again a rise in the transport rate." @default.
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• W2024362885 date "1971-07-01" @default.
• W2024362885 modified "2023-09-24" @default.
• W2024362885 title "Evaluation of kinetic parameters for uptake of amino acids by cells: Effect of insulin on the accumulation of aminoisobutyrate and cycloleucine by isolated rat diaphragm muscle and chick embryo hearts" @default.
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• W2024362885 doi "https://doi.org/10.1016/0005-2736(71)90319-1" @default.
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