Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024365044> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2024365044 endingPage "123" @default.
- W2024365044 startingPage "119" @default.
- W2024365044 abstract "ATP-binding cassette (ABC) transporters extrude a wide variety of endogenous and exogenous compounds. In cancer cells, they are known to confer multidrug resistance. The aim of the present study was to determine the expression of the multidrug resistance-associated protein 1 (MRP1) and 2 (MRP2), which are members of the subfamily C of the ABC transporters family, in human hematopoietic cells.CD4+, CD8+, CD19+, and CD56+ cells were isolated from whole blood by FACS-sort in 20 healthy volunteers. MRP1 and MRP2 mRNA levels were quantified using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assays on a LightCycler (Roche, Mannheim, Germany).The MRP1 mRNA exhibited the highest abundance in CD4+ cells (7.4 x 10(3)+/-3.19 x 10(3) molecules/ng of total RNA), followed by CD8+ > CD19+ > CD56+ cells. The MRP2 mRNA expression was highest in CD4+ cells (6.7 x 10(2)+/-2.84 x 10(2)), followed by CD8+ > CD56+ > CD19+ cells. No correlation between the MRP1 and MRP2 mRNA expression was observed. Interestingly, beta2-microglobulin mRNA expression in CD19+ cells was found to be twofold lower in comparison with other cells.On an mRNA level both MRP1 and MRP2 were expressed in peripheral blood cells, with more than sevenfold higher MRP1 expression in all cell populations investigated. The impact of the MRP1 and MRP2 transcription in these cells remains to study. The use of beta2-microglobulin as a housekeeping gene could have a critical impact on the interpretation of RT-PCR data." @default.
- W2024365044 created "2016-06-24" @default.
- W2024365044 creator A5001414279 @default.
- W2024365044 creator A5025993995 @default.
- W2024365044 creator A5040183274 @default.
- W2024365044 creator A5057268469 @default.
- W2024365044 date "2003-07-22" @default.
- W2024365044 modified "2023-10-15" @default.
- W2024365044 title "Quantitative determination of the human MRP1 and MRP2 mRNA expression in FACS-sorted peripheral blood CD4<sup>+</sup>, CD8<sup>+</sup>, CD19<sup>+</sup>, and CD56<sup>+</sup>cells" @default.
- W2024365044 cites W1502426706 @default.
- W2024365044 cites W1577577364 @default.
- W2024365044 cites W1965129145 @default.
- W2024365044 cites W1968351927 @default.
- W2024365044 cites W2027947014 @default.
- W2024365044 cites W2030449390 @default.
- W2024365044 cites W2066590212 @default.
- W2024365044 cites W2093172276 @default.
- W2024365044 cites W2101254476 @default.
- W2024365044 cites W2109189857 @default.
- W2024365044 cites W2123596072 @default.
- W2024365044 cites W2160246216 @default.
- W2024365044 cites W2168070336 @default.
- W2024365044 cites W2280630460 @default.
- W2024365044 cites W2322143727 @default.
- W2024365044 cites W3111650914 @default.
- W2024365044 doi "https://doi.org/10.1034/j.1600-0609.2003.00100.x" @default.
- W2024365044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12890151" @default.
- W2024365044 hasPublicationYear "2003" @default.
- W2024365044 type Work @default.
- W2024365044 sameAs 2024365044 @default.
- W2024365044 citedByCount "36" @default.
- W2024365044 countsByYear W20243650442012 @default.
- W2024365044 countsByYear W20243650442013 @default.
- W2024365044 countsByYear W20243650442014 @default.
- W2024365044 countsByYear W20243650442015 @default.
- W2024365044 countsByYear W20243650442016 @default.
- W2024365044 countsByYear W20243650442019 @default.
- W2024365044 countsByYear W20243650442020 @default.
- W2024365044 countsByYear W20243650442021 @default.
- W2024365044 crossrefType "journal-article" @default.
- W2024365044 hasAuthorship W2024365044A5001414279 @default.
- W2024365044 hasAuthorship W2024365044A5025993995 @default.
- W2024365044 hasAuthorship W2024365044A5040183274 @default.
- W2024365044 hasAuthorship W2024365044A5057268469 @default.
- W2024365044 hasConcept C104317684 @default.
- W2024365044 hasConcept C105580179 @default.
- W2024365044 hasConcept C147483822 @default.
- W2024365044 hasConcept C149011108 @default.
- W2024365044 hasConcept C153911025 @default.
- W2024365044 hasConcept C167672396 @default.
- W2024365044 hasConcept C203014093 @default.
- W2024365044 hasConcept C2778957590 @default.
- W2024365044 hasConcept C36201501 @default.
- W2024365044 hasConcept C44312359 @default.
- W2024365044 hasConcept C553184892 @default.
- W2024365044 hasConcept C55493867 @default.
- W2024365044 hasConcept C86803240 @default.
- W2024365044 hasConceptScore W2024365044C104317684 @default.
- W2024365044 hasConceptScore W2024365044C105580179 @default.
- W2024365044 hasConceptScore W2024365044C147483822 @default.
- W2024365044 hasConceptScore W2024365044C149011108 @default.
- W2024365044 hasConceptScore W2024365044C153911025 @default.
- W2024365044 hasConceptScore W2024365044C167672396 @default.
- W2024365044 hasConceptScore W2024365044C203014093 @default.
- W2024365044 hasConceptScore W2024365044C2778957590 @default.
- W2024365044 hasConceptScore W2024365044C36201501 @default.
- W2024365044 hasConceptScore W2024365044C44312359 @default.
- W2024365044 hasConceptScore W2024365044C553184892 @default.
- W2024365044 hasConceptScore W2024365044C55493867 @default.
- W2024365044 hasConceptScore W2024365044C86803240 @default.
- W2024365044 hasIssue "2" @default.
- W2024365044 hasLocation W20243650441 @default.
- W2024365044 hasLocation W20243650442 @default.
- W2024365044 hasOpenAccess W2024365044 @default.
- W2024365044 hasPrimaryLocation W20243650441 @default.
- W2024365044 hasRelatedWork W1511140234 @default.
- W2024365044 hasRelatedWork W1975665094 @default.
- W2024365044 hasRelatedWork W1981030836 @default.
- W2024365044 hasRelatedWork W2081213990 @default.
- W2024365044 hasRelatedWork W2084769078 @default.
- W2024365044 hasRelatedWork W2112820588 @default.
- W2024365044 hasRelatedWork W2123467745 @default.
- W2024365044 hasRelatedWork W2127444056 @default.
- W2024365044 hasRelatedWork W2206058523 @default.
- W2024365044 hasRelatedWork W39153851 @default.
- W2024365044 hasVolume "71" @default.
- W2024365044 isParatext "false" @default.
- W2024365044 isRetracted "false" @default.
- W2024365044 magId "2024365044" @default.
- W2024365044 workType "article" @default.