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• W2024378407 abstract "The effect of specific chemical functionalities on the adhesion of polymorphonuclear leukocytes (PMNs) under flow was investigated using a set of well-characterized, chemically functionalized surfaces prepared by self-assembly of alkanethiolate monolayers on gold surfaces. Terminal functionalities included CH3, CH2OH, COOH, and (OCH2CH2)3OH groups. A new surface modification was used to incorporate a phosphorylcholine moiety on the hydroxyl-terminated monolayer. Surface modification was verified using contact-angle measurements, ellipsometry, and X-ray photoelectron spectroscopy. Adhesion on the surfaces was studied in the presence and absence of pre-adsorbed fibrinogen. Fibrinogen adsorption on self-assembled monolayers (SAMs) was quantified using radioisotope detection. PMN adhesion was found to be dependent on the monolayer's terminal functionality. Adhesion was higher on the hydrophobic CH3 surface and the polar COOH monolayer. Leukocyte adhesion was least on the phosphorylcholine-rich surface, followed by the ethylene-oxide-containing monolayer. Cell adhesion also was low on the hydrophilic OH monolayer. Attachment was decreased with increasing shear rate, exhibiting a three-fold decrease between 20 and 100 s−1. Fibrinogen adsorption was higher on the CH3 monolayer but comparable for the other four SAMs. Preincubation of the surfaces with fibrinogen decreased adhesion on all SAMs examined. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 291–301, 2000." @default.
• W2024378407 created "2016-06-24" @default.
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• W2024378407 date "2000-06-05" @default.
• W2024378407 modified "2023-10-18" @default.
• W2024378407 title "Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate" @default.
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• W2024378407 doi "https://doi.org/10.1002/(sici)1097-4636(20000605)50:3<291::aid-jbm2>3.0.co;2-1" @default.
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