FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024389301> ?p ?o ?g. }

• W2024389301 endingPage "243" @default.
• W2024389301 startingPage "234" @default.
• W2024389301 abstract "Flow cytometry and DNA binding-specific fluorescent reagents were used to compare the total DNA, G-C, and A-T content of the epimastigote and trypomastigote stages of Trypanosoma cruzi stocks. Significant total DNA differences of 2–12% between epimastigotes and trypomastigotes were found in three of six stocks studied. The epimastigote G-C content of five of six stocks was 4–8% higher than trypomastigotes, whereas the trypomastigote A-T content was 2.5–13% higher than the epimastigote A-T content. Although no obvious developmental stage association between total DNA and base composition was found, intrastage associations do exist. These observations were unaffected by nucleoprotein extraction implying that the observed differences between trypomastigotes and epimastigotes are not a consequence of nucleoprotein interference with DNA-binding fluorochromes. The nuclei and kinetoplasts of four T. cruzi stocks were isolated and analyzed. Developmental stage differences in nuclear and kinetoplast DNA are stock-dependent and base composition-dependent; both organelles contribute to the observed differences in DNA of intact cells. We found a nearly linear association between the percentage of total kinetoplast DNA, G-C, and A-T content. During metacyclogenesis, the G-C content decreases by approximately 7% as epimastigotes transform into metacyclic trypomastigotes. The decrease in G-C content precedes changes in morphology or in complement resistance. If the DNA changes are causally connected to developmental stage transformations in T. cruzi remains to be determined. However, our results could facilitate studies of the molecular genetic processes the parasite uses to successfully complete various phases of its life cycle and, consequently, the disease process it evokes." @default.
• W2024389301 created "2016-06-24" @default.
• W2024389301 creator A5030734026 @default.
• W2024389301 creator A5068229583 @default.
• W2024389301 date "1991-05-01" @default.
• W2024389301 modified "2023-10-16" @default.
• W2024389301 title "<i>Trypanosoma cruzi:</i>Flow Cytometric Analysis of Developmental Stage Differences in DNA" @default.
• W2024389301 cites W1590106897 @default.
• W2024389301 cites W1605384800 @default.
• W2024389301 cites W1965093302 @default.
• W2024389301 cites W1965899188 @default.
• W2024389301 cites W1972220947 @default.
• W2024389301 cites W1974593679 @default.
• W2024389301 cites W1974866353 @default.
• W2024389301 cites W1977742263 @default.
• W2024389301 cites W1979024863 @default.
• W2024389301 cites W1992760440 @default.
• W2024389301 cites W1992894983 @default.
• W2024389301 cites W1994667816 @default.
• W2024389301 cites W1995386269 @default.
• W2024389301 cites W2000507535 @default.
• W2024389301 cites W2002038501 @default.
• W2024389301 cites W2004773727 @default.
• W2024389301 cites W2011218324 @default.
• W2024389301 cites W2013019342 @default.
• W2024389301 cites W2025444976 @default.
• W2024389301 cites W2028576996 @default.
• W2024389301 cites W2033368604 @default.
• W2024389301 cites W2033566365 @default.
• W2024389301 cites W2037136121 @default.
• W2024389301 cites W2037839940 @default.
• W2024389301 cites W2042885409 @default.
• W2024389301 cites W2047078732 @default.
• W2024389301 cites W2053950751 @default.
• W2024389301 cites W2055213294 @default.
• W2024389301 cites W2056582402 @default.
• W2024389301 cites W2063214816 @default.
• W2024389301 cites W2068867787 @default.
• W2024389301 cites W2072283543 @default.
• W2024389301 cites W2082071395 @default.
• W2024389301 cites W2087142903 @default.
• W2024389301 cites W2088556598 @default.
• W2024389301 cites W2119481376 @default.
• W2024389301 cites W2137360605 @default.
• W2024389301 cites W2167385634 @default.
• W2024389301 cites W2300778955 @default.
• W2024389301 cites W301932538 @default.
• W2024389301 doi "https://doi.org/10.1111/j.1550-7408.1991.tb04435.x" @default.
• W2024389301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1880761" @default.
• W2024389301 hasPublicationYear "1991" @default.
• W2024389301 type Work @default.
• W2024389301 sameAs 2024389301 @default.
• W2024389301 citedByCount "40" @default.
• W2024389301 countsByYear W20243893012015 @default.
• W2024389301 countsByYear W20243893012018 @default.
• W2024389301 crossrefType "journal-article" @default.
• W2024389301 hasAuthorship W2024389301A5030734026 @default.
• W2024389301 hasAuthorship W2024389301A5068229583 @default.
• W2024389301 hasConcept C104317684 @default.
• W2024389301 hasConcept C115960442 @default.
• W2024389301 hasConcept C136764020 @default.
• W2024389301 hasConcept C153911025 @default.
• W2024389301 hasConcept C168240541 @default.
• W2024389301 hasConcept C24586158 @default.
• W2024389301 hasConcept C2776027055 @default.
• W2024389301 hasConcept C2777625466 @default.
• W2024389301 hasConcept C2779121891 @default.
• W2024389301 hasConcept C41008148 @default.
• W2024389301 hasConcept C552990157 @default.
• W2024389301 hasConcept C553184892 @default.
• W2024389301 hasConcept C55493867 @default.
• W2024389301 hasConcept C71928629 @default.
• W2024389301 hasConcept C86803240 @default.
• W2024389301 hasConcept C95444343 @default.
• W2024389301 hasConceptScore W2024389301C104317684 @default.
• W2024389301 hasConceptScore W2024389301C115960442 @default.
• W2024389301 hasConceptScore W2024389301C136764020 @default.
• W2024389301 hasConceptScore W2024389301C153911025 @default.
• W2024389301 hasConceptScore W2024389301C168240541 @default.
• W2024389301 hasConceptScore W2024389301C24586158 @default.
• W2024389301 hasConceptScore W2024389301C2776027055 @default.
• W2024389301 hasConceptScore W2024389301C2777625466 @default.
• W2024389301 hasConceptScore W2024389301C2779121891 @default.
• W2024389301 hasConceptScore W2024389301C41008148 @default.
• W2024389301 hasConceptScore W2024389301C552990157 @default.
• W2024389301 hasConceptScore W2024389301C553184892 @default.
• W2024389301 hasConceptScore W2024389301C55493867 @default.
• W2024389301 hasConceptScore W2024389301C71928629 @default.
• W2024389301 hasConceptScore W2024389301C86803240 @default.
• W2024389301 hasConceptScore W2024389301C95444343 @default.
• W2024389301 hasIssue "3" @default.
• W2024389301 hasLocation W20243893011 @default.
• W2024389301 hasLocation W20243893012 @default.
• W2024389301 hasOpenAccess W2024389301 @default.
• W2024389301 hasPrimaryLocation W20243893011 @default.
• W2024389301 hasRelatedWork W1509470394 @default.
• W2024389301 hasRelatedWork W1964837864 @default.
• W2024389301 hasRelatedWork W2039500780 @default.

• next