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• W2024404866 abstract "Spectrophotometric and kinetic measurements were applied to yield photosystem (PS) stoichiometries and the functional antenna size of PSI, PSIIα, and PSIIβ in Zea mays chloroplasts in situ. Concentrations of PSII and PSI reaction centers were determined from the amplitude of the light-induced absorbance change at 320 and 700 nm, which reflect the photoreduction of the primary electron acceptor Q of PSII and the photooxidation of the reaction center P700 of PSI, respectively. Determination of the functional chlorophyll antenna size (N) for each photosystem was obtained from the measurement of the rate of light absorption by the respective reaction center. Under the experimental conditions employed, the rate of light absorption by each reaction center was directly proportional to the number of light-harvesting chlorophyll molecules associated with the respective photosystem. We determined NP700 = 195, Nα = 230, Nβ = 50 for the number of chlorophyll molecules in the light-harvesting antenna of PSI, PSIIα, and PSIIβ, respectively. The above values were used to estimate the PSII/PSI electron-transport capacity ratio (C) in maize chloroplasts. In mesophyll chloroplasts C > 1.4, indicating that, under green actinic excitation when Chl a and Chl b molecules absorb nearly equal amounts of excitation, PSII has a capacity to turn over electrons faster than PSI. In bundle sheath chloroplasts C < 1, suggesting that such chloroplasts are not optimally poised for linear electron transport and reductant generation." @default.
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• W2024404866 date "1984-04-01" @default.
• W2024404866 modified "2023-10-16" @default.
• W2024404866 title "Photosystem Electron-Transport Capacity and Light-Harvesting Antenna Size in Maize Chloroplasts" @default.
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• W2024404866 doi "https://doi.org/10.1104/pp.74.4.993" @default.
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