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• W2024406977 abstract "The effect of a tumor promoter, 12-0-tetradecanoyl phorbol-13-acetate (TPA), on the expression of myosin heavy chain isoforms in cultured rat cardiac ventiricular muscle cells was studied. The previous preliminary report [Claycomb WC (1988): “Biology of Isolated Adult Cardiac Myocytes.” In Clark WA, Decker RS, Borg TK (eds): New York: Elsevier, pp 284–287] indicated that TPA turns off the expression of myosin heavy chain genes in cultured adult cardiac myocytes. Electrophoretic and immunocytochemical analyses were carried out in the present studies. The myosin heavy chain isoform profiles of cardiac myocytes exposed to TPA at concentrations of 50–250 ng/ml culture medium for varying periods were similar to those of controls that were grown in the absence of TPA, showing predominant isoform V1. Immunofluorescence microscopy with monoclonal antibodies to cardiac ventricular isomyosin revealed the structural organization of myosin in TPA-treated cells. The organization of myosin was variable among different myocytes and within a single myocyte. Immunofluorescence microscopy was extended to the examination of the organization of α-actinin which did not differ from that of myosin in some myocytes. In contrast to the previous report [Claycomb, 1988], this study has demonstrated that TPA has no influence on the expression of myosin heavy chain isoforms in cultured adult ventricular cardiac muscle cells." @default.
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• W2024406977 date "1992-08-01" @default.
• W2024406977 modified "2023-10-18" @default.
• W2024406977 title "TPA has no influence on the expression of myosin heavy chain isoforms in cultured adult cardiac muscle cells" @default.
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• W2024406977 doi "https://doi.org/10.1002/jcb.240490410" @default.
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