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• W2024407294 abstract "The superfamily 1 helicase, RecD2, is a monomeric, bacterial enzyme with a role in DNA repair, but with 5'-3' activity unlike most enzymes from this superfamily. Rate constants were determined for steps within the ATPase cycle of RecD2 in the presence of ssDNA. The fluorescent ATP analog, mantATP (2'(3')-O-(N-methylanthraniloyl)ATP), was used throughout to provide a complete set of rate constants and determine the mechanism of the cycle for a single nucleotide species. Fluorescence stopped-flow measurements were used to determine rate constants for adenosine nucleotide binding and release, quenched-flow measurements were used for the hydrolytic cleavage step, and the fluorescent phosphate biosensor was used for phosphate release kinetics. Some rate constants could also be measured using the natural substrate, ATP, and these suggested a similar mechanism to that obtained with mantATP. The data show that a rearrangement linked to Mg(2+) coordination, which occurs before the hydrolysis step, is rate-limiting in the cycle and that this step is greatly accelerated by bound DNA. This is also shown here for the PcrA 3'-5' helicase and so may be a general mechanism governing superfamily 1 helicases. The mechanism accounts for the tight coupling between translocation and ATPase activity." @default.
• W2024407294 created "2016-06-24" @default.
• W2024407294 creator A5051163780 @default.
• W2024407294 creator A5078281981 @default.
• W2024407294 date "2013-08-01" @default.
• W2024407294 modified "2023-10-13" @default.
• W2024407294 title "ATPase Mechanism of the 5′-3′ DNA Helicase, RecD2" @default.
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• W2024407294 doi "https://doi.org/10.1074/jbc.m113.484667" @default.
• W2024407294 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3757182" @default.
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