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• W2024421175 endingPage "3128" @default.
• W2024421175 startingPage "3120" @default.
• W2024421175 abstract "Abstract Our laboratory recently identified a quiescent class of pluripotent hematopoietic stem cells (PHSCs) that are lineage negative (Linneg), lack c-Kit, and are able to give rise to c-Kit–positive (c-Kitpos) PHSCs in vivo. This population fails to proliferate in vitro but has delayed reconstituting activity in vivo. In this study, we purified these cells to enrich for the PHSCs and we identified in vitro conditions capable of supporting their maturation. The c-Kit–negative (c-Kitneg) cells exhibited differential expression of Sca-1, CD34, CD43, CD45, and Thy 1.2. We purified the cells based on Sca-1, as it is expressed on active PHSCs. We detected pre–colony-forming unit spleen (pre–CFU-s) activity in both the Sca-1neg and Sca-1pos populations, indicating the presence of primitive PHSCs in both populations. However, our in vitro studies suggest that the Sca-1pos population is enriched for PHSCs. The in vitro systems that support the growth of these dormant cells include a modified long-term marrow culture and various stromal cell lines. In modified long-term bone marrow cultures, c-Kitneg cells gave rise to c-Kitpos PHSCs, with long-term reconstitution activity in vivo. Thus we have established an in vitro system to examine PHSC maturation that will allow us to study the mediators of the c-Kitneg to c-Kitpos transition." @default.
• W2024421175 created "2016-06-24" @default.
• W2024421175 creator A5041498573 @default.
• W2024421175 creator A5057465076 @default.
• W2024421175 creator A5075594448 @default.
• W2024421175 creator A5090696791 @default.
• W2024421175 date "2003-11-01" @default.
• W2024421175 modified "2023-09-26" @default.
• W2024421175 title "Identification of in vitro growth conditions for c-Kit–negative hematopoietic stem cells" @default.
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• W2024421175 doi "https://doi.org/10.1182/blood-2003-04-1249" @default.
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