Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024433050> ?p ?o ?g. }
- W2024433050 endingPage "1732" @default.
- W2024433050 startingPage "1725" @default.
- W2024433050 abstract "Pseudomonas strain LB400 is able to degrade an unusually wide variety of polychlorinated biphenyls (PCBs). A genomic library of LB400 was constructed by using the broad-host-range cosmid pMMB34 and introduced into Escherichia coli. Approximately 1,600 recombinant clones were tested, and 5 that expressed 2,3-dihydroxybiphenyl dioxygenase activity were found. This enzyme is encoded by the bphC gene of the 2,3-dioxygenase pathway for PCB-biphenyl metabolism. Two recombinant plasmids encoding the ability to transform PCBs to chlorobenzoic acids were identified, and one of these, pGEM410, was chosen for further study. The PCB-degrading genes (bphA, -B, -C, and -D) were localized by subcloning experiments to a 12.4-kilobase region of pGEM410. The ability of recombinant strains to degrade PCBs was compared with that of the wild type. In resting-cell assays, PCB degradation by E. coli strain FM4560 (containing a pGEM410 derivative) approached that of LB400 and was significantly greater than degradation by the original recombinant strain. High levels of PCB metabolism by FM4560 did not depend on the growth of the organism on biphenyl, as it did for PCB metabolism by LB400. When cells were grown with succinate as the carbon source, PCB degradation by FM4560 was markedly superior to that by LB400." @default.
- W2024433050 created "2016-06-24" @default.
- W2024433050 creator A5023011044 @default.
- W2024433050 date "1989-03-01" @default.
- W2024433050 modified "2023-09-26" @default.
- W2024433050 title "Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation" @default.
- W2024433050 cites W1511459442 @default.
- W2024433050 cites W1521081413 @default.
- W2024433050 cites W1526138621 @default.
- W2024433050 cites W1592048772 @default.
- W2024433050 cites W1594688023 @default.
- W2024433050 cites W1608761244 @default.
- W2024433050 cites W1818468124 @default.
- W2024433050 cites W1934359967 @default.
- W2024433050 cites W1957969272 @default.
- W2024433050 cites W1974303188 @default.
- W2024433050 cites W197854464 @default.
- W2024433050 cites W1999265155 @default.
- W2024433050 cites W2002685115 @default.
- W2024433050 cites W2011928243 @default.
- W2024433050 cites W2035155772 @default.
- W2024433050 cites W2036437888 @default.
- W2024433050 cites W2063450941 @default.
- W2024433050 cites W2073892189 @default.
- W2024433050 cites W2129349667 @default.
- W2024433050 cites W2156402236 @default.
- W2024433050 cites W2409064786 @default.
- W2024433050 cites W2463267938 @default.
- W2024433050 cites W2907416725 @default.
- W2024433050 cites W4296765338 @default.
- W2024433050 doi "https://doi.org/10.1128/jb.171.3.1725-1732.1989" @default.
- W2024433050 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/209804" @default.
- W2024433050 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2493454" @default.
- W2024433050 hasPublicationYear "1989" @default.
- W2024433050 type Work @default.
- W2024433050 sameAs 2024433050 @default.
- W2024433050 citedByCount "195" @default.
- W2024433050 countsByYear W20244330502012 @default.
- W2024433050 countsByYear W20244330502013 @default.
- W2024433050 countsByYear W20244330502015 @default.
- W2024433050 countsByYear W20244330502016 @default.
- W2024433050 countsByYear W20244330502017 @default.
- W2024433050 countsByYear W20244330502019 @default.
- W2024433050 countsByYear W20244330502020 @default.
- W2024433050 countsByYear W20244330502021 @default.
- W2024433050 countsByYear W20244330502023 @default.
- W2024433050 crossrefType "journal-article" @default.
- W2024433050 hasAuthorship W2024433050A5023011044 @default.
- W2024433050 hasBestOaLocation W20244330502 @default.
- W2024433050 hasConcept C104317684 @default.
- W2024433050 hasConcept C105702510 @default.
- W2024433050 hasConcept C150194340 @default.
- W2024433050 hasConcept C153911025 @default.
- W2024433050 hasConcept C163005120 @default.
- W2024433050 hasConcept C19924922 @default.
- W2024433050 hasConcept C22744801 @default.
- W2024433050 hasConcept C2776834422 @default.
- W2024433050 hasConcept C2778022156 @default.
- W2024433050 hasConcept C2781463711 @default.
- W2024433050 hasConcept C35101798 @default.
- W2024433050 hasConcept C40767141 @default.
- W2024433050 hasConcept C523546767 @default.
- W2024433050 hasConcept C54355233 @default.
- W2024433050 hasConcept C547475151 @default.
- W2024433050 hasConcept C55493867 @default.
- W2024433050 hasConcept C86803240 @default.
- W2024433050 hasConcept C89423630 @default.
- W2024433050 hasConceptScore W2024433050C104317684 @default.
- W2024433050 hasConceptScore W2024433050C105702510 @default.
- W2024433050 hasConceptScore W2024433050C150194340 @default.
- W2024433050 hasConceptScore W2024433050C153911025 @default.
- W2024433050 hasConceptScore W2024433050C163005120 @default.
- W2024433050 hasConceptScore W2024433050C19924922 @default.
- W2024433050 hasConceptScore W2024433050C22744801 @default.
- W2024433050 hasConceptScore W2024433050C2776834422 @default.
- W2024433050 hasConceptScore W2024433050C2778022156 @default.
- W2024433050 hasConceptScore W2024433050C2781463711 @default.
- W2024433050 hasConceptScore W2024433050C35101798 @default.
- W2024433050 hasConceptScore W2024433050C40767141 @default.
- W2024433050 hasConceptScore W2024433050C523546767 @default.
- W2024433050 hasConceptScore W2024433050C54355233 @default.
- W2024433050 hasConceptScore W2024433050C547475151 @default.
- W2024433050 hasConceptScore W2024433050C55493867 @default.
- W2024433050 hasConceptScore W2024433050C86803240 @default.
- W2024433050 hasConceptScore W2024433050C89423630 @default.
- W2024433050 hasIssue "3" @default.
- W2024433050 hasLocation W20244330501 @default.
- W2024433050 hasLocation W20244330502 @default.
- W2024433050 hasLocation W20244330503 @default.
- W2024433050 hasLocation W20244330504 @default.
- W2024433050 hasOpenAccess W2024433050 @default.
- W2024433050 hasPrimaryLocation W20244330501 @default.
- W2024433050 hasRelatedWork W1621677732 @default.
- W2024433050 hasRelatedWork W1927433050 @default.
- W2024433050 hasRelatedWork W1969249387 @default.
- W2024433050 hasRelatedWork W1972010400 @default.
- W2024433050 hasRelatedWork W1985426194 @default.
- W2024433050 hasRelatedWork W1988049223 @default.