FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024444798> ?p ?o ?g. }

• W2024444798 endingPage "1460" @default.
• W2024444798 startingPage "1450" @default.
• W2024444798 abstract "In the absence of mechanical stimulation, brief exposure of osteoblasts to 1alpha,25(OH)(2)vitamin D(3) (1,25D) triggers plasma membrane electrical responses that couple to exocytosis. Here we describe for the first time 1,25D induction of exocytotic ATP release in static ROS 17/2.8 and SAOS-2 cells and primary calvarial osteoblasts expressing a vitamin D receptor (VDR). We found that 10 nM 1,25D optimally induced 45 +/- 1% and 40 +/- 1% of partial and complete exocytotic events, respectively, from a 1,25D-sensitive pool of ATP-containing secretory vesicles within 60 s. We measured a dose-dependent 1,25D induction of ATP secretion, with maximal response of approximately 6.2-fold (16.93 +/- 1.82 nM for SAOS-2) and 3.1-fold (18.89 +/- 1.39 nM for ROS 17/2.8) obtained with 10 nM 1,25D compared with basal ATP levels (2.75 +/- 0.39 nM, SAOS-2; 6.09 +/- 0.58 nM, ROS 17/2.8 cells). The natural metabolite 25(OH)vitamin D(3) (25D, 10 nM) induced a significant 3.6-fold increase of ATP release in ROS 17/2.8 cells, but there was no induction with the antagonist 1beta,25(OH)(2)vitamin D(3) (1beta,25D, 10 nM) or the steroid 17beta-estradiol (10 nM). 1,25D-induced ATP secretion was abolished when cells were preincubated with inhibitors of vesicular exocytosis. siRNA VDR silencing prevented 1,25D stimulation of ATP exocytosis in ROS 17/2.8 and SAOS-2 cells. Similarly, 1,25D failed to stimulate ATP exocytosis in primary osteoblasts from a VDR knockout mouse. ATP secretion coupled to 1,25D induction of cytosolic calcium and chloride channel potentiation. Rapid 1,25D stimulation of ATP secretion involving nontranscriptional VDR functions in osteoblasts may help explain 1,25D bone anabolic properties." @default.
• W2024444798 created "2016-06-24" @default.
• W2024444798 creator A5003197117 @default.
• W2024444798 creator A5021196814 @default.
• W2024444798 date "2009-08-01" @default.
• W2024444798 modified "2023-10-14" @default.
• W2024444798 title "1α,25(OH)₂Vitamin D₃Induction of ATP Secretion in Osteoblasts" @default.
• W2024444798 cites W1576923208 @default.
• W2024444798 cites W1584929737 @default.
• W2024444798 cites W1827847463 @default.
• W2024444798 cites W1965470981 @default.
• W2024444798 cites W1968834775 @default.
• W2024444798 cites W1976958448 @default.
• W2024444798 cites W1980144849 @default.
• W2024444798 cites W1981919734 @default.
• W2024444798 cites W1986454463 @default.
• W2024444798 cites W2000956764 @default.
• W2024444798 cites W2003980854 @default.
• W2024444798 cites W2015839100 @default.
• W2024444798 cites W2016506774 @default.
• W2024444798 cites W2016911103 @default.
• W2024444798 cites W2018856950 @default.
• W2024444798 cites W2024881682 @default.
• W2024444798 cites W2026264562 @default.
• W2024444798 cites W2037816904 @default.
• W2024444798 cites W2042261922 @default.
• W2024444798 cites W2044028474 @default.
• W2024444798 cites W2052109566 @default.
• W2024444798 cites W2055532277 @default.
• W2024444798 cites W2066227639 @default.
• W2024444798 cites W2078621767 @default.
• W2024444798 cites W2087890771 @default.
• W2024444798 cites W2090464853 @default.
• W2024444798 cites W2111550283 @default.
• W2024444798 cites W2119600321 @default.
• W2024444798 cites W2123416020 @default.
• W2024444798 cites W2126153001 @default.
• W2024444798 cites W2132311956 @default.
• W2024444798 cites W2137780990 @default.
• W2024444798 cites W2138599948 @default.
• W2024444798 cites W2157463004 @default.
• W2024444798 cites W2169314089 @default.
• W2024444798 cites W2381492668 @default.
• W2024444798 doi "https://doi.org/10.1359/jbmr.090306" @default.
• W2024444798 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2718799" @default.
• W2024444798 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19292616" @default.
• W2024444798 hasPublicationYear "2009" @default.
• W2024444798 type Work @default.
• W2024444798 sameAs 2024444798 @default.
• W2024444798 citedByCount "26" @default.
• W2024444798 countsByYear W20244447982012 @default.
• W2024444798 countsByYear W20244447982013 @default.
• W2024444798 countsByYear W20244447982014 @default.
• W2024444798 countsByYear W20244447982015 @default.
• W2024444798 countsByYear W20244447982016 @default.
• W2024444798 countsByYear W20244447982018 @default.
• W2024444798 countsByYear W20244447982020 @default.
• W2024444798 countsByYear W20244447982021 @default.
• W2024444798 countsByYear W20244447982023 @default.
• W2024444798 crossrefType "journal-article" @default.
• W2024444798 hasAuthorship W2024444798A5003197117 @default.
• W2024444798 hasAuthorship W2024444798A5021196814 @default.
• W2024444798 hasBestOaLocation W20244447982 @default.
• W2024444798 hasConcept C124490489 @default.
• W2024444798 hasConcept C126322002 @default.
• W2024444798 hasConcept C134018914 @default.
• W2024444798 hasConcept C175969161 @default.
• W2024444798 hasConcept C179639408 @default.
• W2024444798 hasConcept C185592680 @default.
• W2024444798 hasConcept C24998067 @default.
• W2024444798 hasConcept C2777477808 @default.
• W2024444798 hasConcept C49039625 @default.
• W2024444798 hasConcept C71924100 @default.
• W2024444798 hasConcept C86803240 @default.
• W2024444798 hasConcept C95444343 @default.
• W2024444798 hasConceptScore W2024444798C124490489 @default.
• W2024444798 hasConceptScore W2024444798C126322002 @default.
• W2024444798 hasConceptScore W2024444798C134018914 @default.
• W2024444798 hasConceptScore W2024444798C175969161 @default.
• W2024444798 hasConceptScore W2024444798C179639408 @default.
• W2024444798 hasConceptScore W2024444798C185592680 @default.
• W2024444798 hasConceptScore W2024444798C24998067 @default.
• W2024444798 hasConceptScore W2024444798C2777477808 @default.
• W2024444798 hasConceptScore W2024444798C49039625 @default.
• W2024444798 hasConceptScore W2024444798C71924100 @default.
• W2024444798 hasConceptScore W2024444798C86803240 @default.
• W2024444798 hasConceptScore W2024444798C95444343 @default.
• W2024444798 hasIssue "8" @default.
• W2024444798 hasLocation W20244447981 @default.
• W2024444798 hasLocation W20244447982 @default.
• W2024444798 hasLocation W20244447983 @default.
• W2024444798 hasLocation W20244447984 @default.
• W2024444798 hasOpenAccess W2024444798 @default.
• W2024444798 hasPrimaryLocation W20244447981 @default.
• W2024444798 hasRelatedWork W170075149 @default.
• W2024444798 hasRelatedWork W2000797165 @default.
• W2024444798 hasRelatedWork W2011197845 @default.
• W2024444798 hasRelatedWork W2055335988 @default.

• next