FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024446308> ?p ?o ?g. }

• W2024446308 endingPage "1459" @default.
• W2024446308 startingPage "1449" @default.
• W2024446308 abstract "To learn more about the possible role of the coated pits endocytic pathway in cell adhesion, we studied attachment and spreading of fibroblasts whose coated pits were disrupted by depletion of intercellular potassium. Fibroblasts incubated in suspension in potassium-free medium lost 80% of their intracellular potassium within 10 min and showed disrupted coated pits based on fluorescence staining of clathrin. Potassium-depleted cells attached and spread on fibronectin-coated substrata over the same time course (15 min-2 h) as control cells. Unlike controls, however, potassium-depleted fibroblasts attained a radial morphology with circumferentially organized actin filament bundles and were unable to make the transition to a polarized morphology with stress fibers. In the radially spread fibroblasts, fibronectin receptors and vinculin colocalized in focal adhesion sites and appeared to be membrane insertion points for circumferentially arranged actin filament bundles, but these sites were much smaller than the focal adhesion plaques in polarized cells. The effects of potassium depletion on cell adhesion were reversible. Within 1 h after switching K(+)-depleted fibroblasts to medium containing KCl, cells developed a polarized morphology with actin stress fibers inserting into focal adhesion plaques. Coated pits also reformed on the cell surface during this time. Because formation of focal adhesion plaques preceded reappearance of clathrin-coated pits at the cell margins, it seems unlikely that coated pits play a direct role in adhesion plaque assembly. Polarization of fibroblasts upon addition of KCl was inhibited by ouabain showing that intracellular potassium was required for activity. Polarization also was inhibited when potassium-depleted cells were switched to potassium-containing medium under hypertonic or acidified conditions, both of which have been shown to inhibit receptor-mediated endocytosis. Our results suggest that the coated pit endocytic pathway is not required for initial attachment, spreading, and formation of focal adhesions by fibroblasts, but may play a role in cell polarization." @default.
• W2024446308 created "2016-06-24" @default.
• W2024446308 creator A5043692232 @default.
• W2024446308 creator A5076643156 @default.
• W2024446308 date "1993-03-15" @default.
• W2024446308 modified "2023-09-26" @default.
• W2024446308 title "Depletion of intracellular potassium disrupts coated pits and reversibly inhibits cell polarization during fibroblast spreading." @default.
• W2024446308 cites W147102809 @default.
• W2024446308 cites W1487575968 @default.
• W2024446308 cites W1506805743 @default.
• W2024446308 cites W1607784649 @default.
• W2024446308 cites W179550893 @default.
• W2024446308 cites W1806064940 @default.
• W2024446308 cites W1964737074 @default.
• W2024446308 cites W1966286149 @default.
• W2024446308 cites W1979825747 @default.
• W2024446308 cites W1987568997 @default.
• W2024446308 cites W1994462082 @default.
• W2024446308 cites W1997417732 @default.
• W2024446308 cites W1999393451 @default.
• W2024446308 cites W2006526726 @default.
• W2024446308 cites W2010381093 @default.
• W2024446308 cites W2013381560 @default.
• W2024446308 cites W2017201573 @default.
• W2024446308 cites W2041534105 @default.
• W2024446308 cites W2049732058 @default.
• W2024446308 cites W2056104802 @default.
• W2024446308 cites W2062576661 @default.
• W2024446308 cites W2063753069 @default.
• W2024446308 cites W2070431772 @default.
• W2024446308 cites W2074492469 @default.
• W2024446308 cites W2075492377 @default.
• W2024446308 cites W2079312655 @default.
• W2024446308 cites W2082478918 @default.
• W2024446308 cites W2083987209 @default.
• W2024446308 cites W2109505295 @default.
• W2024446308 cites W2115876454 @default.
• W2024446308 cites W2135121318 @default.
• W2024446308 cites W2139666587 @default.
• W2024446308 cites W2140622211 @default.
• W2024446308 cites W2148329395 @default.
• W2024446308 cites W2152415000 @default.
• W2024446308 cites W2228492167 @default.
• W2024446308 cites W2332221637 @default.
• W2024446308 cites W2334377491 @default.
• W2024446308 cites W2399310390 @default.
• W2024446308 cites W2404471159 @default.
• W2024446308 cites W2428029072 @default.
• W2024446308 cites W2441332987 @default.
• W2024446308 doi "https://doi.org/10.1083/jcb.120.6.1449" @default.
• W2024446308 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2119743" @default.
• W2024446308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8449988" @default.
• W2024446308 hasPublicationYear "1993" @default.
• W2024446308 type Work @default.
• W2024446308 sameAs 2024446308 @default.
• W2024446308 citedByCount "62" @default.
• W2024446308 countsByYear W20244463082012 @default.
• W2024446308 countsByYear W20244463082013 @default.
• W2024446308 countsByYear W20244463082014 @default.
• W2024446308 countsByYear W20244463082015 @default.
• W2024446308 countsByYear W20244463082017 @default.
• W2024446308 countsByYear W20244463082018 @default.
• W2024446308 countsByYear W20244463082019 @default.
• W2024446308 countsByYear W20244463082020 @default.
• W2024446308 countsByYear W20244463082021 @default.
• W2024446308 countsByYear W20244463082022 @default.
• W2024446308 crossrefType "journal-article" @default.
• W2024446308 hasAuthorship W2024446308A5043692232 @default.
• W2024446308 hasAuthorship W2024446308A5076643156 @default.
• W2024446308 hasBestOaLocation W20244463081 @default.
• W2024446308 hasConcept C12554922 @default.
• W2024446308 hasConcept C125705527 @default.
• W2024446308 hasConcept C142669718 @default.
• W2024446308 hasConcept C1491633281 @default.
• W2024446308 hasConcept C151166631 @default.
• W2024446308 hasConcept C159985019 @default.
• W2024446308 hasConcept C189040839 @default.
• W2024446308 hasConcept C189165786 @default.
• W2024446308 hasConcept C192562407 @default.
• W2024446308 hasConcept C2777979848 @default.
• W2024446308 hasConcept C2780381497 @default.
• W2024446308 hasConcept C54355233 @default.
• W2024446308 hasConcept C55493867 @default.
• W2024446308 hasConcept C62478195 @default.
• W2024446308 hasConcept C79879829 @default.
• W2024446308 hasConcept C81885089 @default.
• W2024446308 hasConcept C84416704 @default.
• W2024446308 hasConcept C85789140 @default.
• W2024446308 hasConcept C86492073 @default.
• W2024446308 hasConcept C86803240 @default.
• W2024446308 hasConcept C95444343 @default.
• W2024446308 hasConceptScore W2024446308C12554922 @default.
• W2024446308 hasConceptScore W2024446308C125705527 @default.
• W2024446308 hasConceptScore W2024446308C142669718 @default.
• W2024446308 hasConceptScore W2024446308C1491633281 @default.
• W2024446308 hasConceptScore W2024446308C151166631 @default.
• W2024446308 hasConceptScore W2024446308C159985019 @default.
• W2024446308 hasConceptScore W2024446308C189040839 @default.

• next