Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024456717> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2024456717 endingPage "7209" @default.
- W2024456717 startingPage "7205" @default.
- W2024456717 abstract "To investigate the role of the leader peptide in modulating secretion from living cells, we injected a synthetic peptide into Xenopus oocytes. The peptide consisted of the NH2-terminal leader sequence of mouse immunoglobulin light chain precursor. We found that the leader peptide has two different roles in regulating secretion from the oocytes. First, it competitively inhibits the synthesis of secretory and membrane proteins but not of cytoplasmic proteins. The inhibition occurs both with oocyte proteins and with proteins directed by coinjected myeloma mRNA. The inhibition reaches a maximum 2 hr after injection and decays within 3 hr. It appears to be mediated through the cell membrane, because 125I-labeled leader peptide segregates into the membrane fraction of microinjected oocytes simultaneously with the interference with methionine incorporation. A second role of the microinjected leader peptide is to induce a rapid acceleration in the rate of export of secretory proteins from the oocyte. The maximal enhancement effect is obtained upon injection of 50 ng of leader peptide per oocyte. It is not merely due to the small size, negative charge, or hydrophobicity of the peptide, because enhanced secretion does not occur when glucagon, poly-L-glutamic acid, or Triton X-100 is injected. Furthermore, immunoreaction of the peptide with specific antibodies prior to microinjection prevents the accelerated export. Our observations indicate that in Xenopus oocytes, the leader peptide is involved in both translocation and later step(s) in the secretory pathway." @default.
- W2024456717 created "2016-06-24" @default.
- W2024456717 creator A5001923252 @default.
- W2024456717 creator A5003680413 @default.
- W2024456717 creator A5024791254 @default.
- W2024456717 date "1983-12-01" @default.
- W2024456717 modified "2023-10-14" @default.
- W2024456717 title "Synthetic leader peptide modulates secretion of proteins from microinjected Xenopus oocytes." @default.
- W2024456717 cites W1484115349 @default.
- W2024456717 cites W1517005261 @default.
- W2024456717 cites W157034127 @default.
- W2024456717 cites W1965316807 @default.
- W2024456717 cites W1965956303 @default.
- W2024456717 cites W1975567952 @default.
- W2024456717 cites W1976558489 @default.
- W2024456717 cites W1993381319 @default.
- W2024456717 cites W1995622671 @default.
- W2024456717 cites W2002699686 @default.
- W2024456717 cites W2008974945 @default.
- W2024456717 cites W2009080452 @default.
- W2024456717 cites W2018289835 @default.
- W2024456717 cites W2023816560 @default.
- W2024456717 cites W2027407556 @default.
- W2024456717 cites W2030685467 @default.
- W2024456717 cites W2044192093 @default.
- W2024456717 cites W2045431978 @default.
- W2024456717 cites W2064377006 @default.
- W2024456717 cites W2066160869 @default.
- W2024456717 cites W2076856886 @default.
- W2024456717 cites W2077947679 @default.
- W2024456717 cites W2082111162 @default.
- W2024456717 cites W2100837269 @default.
- W2024456717 cites W2102070772 @default.
- W2024456717 cites W2108708484 @default.
- W2024456717 cites W2144667682 @default.
- W2024456717 cites W2146923243 @default.
- W2024456717 cites W2171903383 @default.
- W2024456717 cites W2173897585 @default.
- W2024456717 cites W2428551409 @default.
- W2024456717 cites W258376682 @default.
- W2024456717 doi "https://doi.org/10.1073/pnas.80.23.7205" @default.
- W2024456717 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/390023" @default.
- W2024456717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6580639" @default.
- W2024456717 hasPublicationYear "1983" @default.
- W2024456717 type Work @default.
- W2024456717 sameAs 2024456717 @default.
- W2024456717 citedByCount "19" @default.
- W2024456717 crossrefType "journal-article" @default.
- W2024456717 hasAuthorship W2024456717A5001923252 @default.
- W2024456717 hasAuthorship W2024456717A5003680413 @default.
- W2024456717 hasAuthorship W2024456717A5024791254 @default.
- W2024456717 hasBestOaLocation W20244567172 @default.
- W2024456717 hasConcept C104317684 @default.
- W2024456717 hasConcept C10858879 @default.
- W2024456717 hasConcept C167625842 @default.
- W2024456717 hasConcept C196843134 @default.
- W2024456717 hasConcept C2776690073 @default.
- W2024456717 hasConcept C2779281246 @default.
- W2024456717 hasConcept C2779535977 @default.
- W2024456717 hasConcept C49039625 @default.
- W2024456717 hasConcept C55493867 @default.
- W2024456717 hasConcept C62525196 @default.
- W2024456717 hasConcept C86803240 @default.
- W2024456717 hasConcept C95444343 @default.
- W2024456717 hasConceptScore W2024456717C104317684 @default.
- W2024456717 hasConceptScore W2024456717C10858879 @default.
- W2024456717 hasConceptScore W2024456717C167625842 @default.
- W2024456717 hasConceptScore W2024456717C196843134 @default.
- W2024456717 hasConceptScore W2024456717C2776690073 @default.
- W2024456717 hasConceptScore W2024456717C2779281246 @default.
- W2024456717 hasConceptScore W2024456717C2779535977 @default.
- W2024456717 hasConceptScore W2024456717C49039625 @default.
- W2024456717 hasConceptScore W2024456717C55493867 @default.
- W2024456717 hasConceptScore W2024456717C62525196 @default.
- W2024456717 hasConceptScore W2024456717C86803240 @default.
- W2024456717 hasConceptScore W2024456717C95444343 @default.
- W2024456717 hasIssue "23" @default.
- W2024456717 hasLocation W20244567171 @default.
- W2024456717 hasLocation W20244567172 @default.
- W2024456717 hasLocation W20244567173 @default.
- W2024456717 hasLocation W20244567174 @default.
- W2024456717 hasOpenAccess W2024456717 @default.
- W2024456717 hasPrimaryLocation W20244567171 @default.
- W2024456717 hasRelatedWork W1597991663 @default.
- W2024456717 hasRelatedWork W1973975221 @default.
- W2024456717 hasRelatedWork W2002367616 @default.
- W2024456717 hasRelatedWork W2024456717 @default.
- W2024456717 hasRelatedWork W2114284755 @default.
- W2024456717 hasRelatedWork W2205628801 @default.
- W2024456717 hasRelatedWork W2311082910 @default.
- W2024456717 hasRelatedWork W2409720367 @default.
- W2024456717 hasRelatedWork W25064496 @default.
- W2024456717 hasRelatedWork W93852916 @default.
- W2024456717 hasVolume "80" @default.
- W2024456717 isParatext "false" @default.
- W2024456717 isRetracted "false" @default.
- W2024456717 magId "2024456717" @default.
- W2024456717 workType "article" @default.