Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024458667> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2024458667 endingPage "115" @default.
- W2024458667 startingPage "111" @default.
- W2024458667 abstract "Apoptosis is characterized by DNA strand breaks with a 3′-OH terminus, which are analyzed by terminal deoxy(d)-UTP nick end labeling (TUNEL). Proteinase K digestion is thought to be an essential step in the TUNEL procedure. The effects of decalcifying reagents on general staining and the TUNEL assay for cartilage sections are largely unknown. The effects of these reagents on retention and integrity of DNA in chondrocytes have not been described until now. We evaluated the effects of various decalcifying solutions, including 10% EDTA, 10% citric acid, 5% trichloroacetic acid, 5% acetic acid and a commercial hydrochloric acid-based reagent, on general cartilage staining and the TUNEL assay for cartilage. The effects of proteinase K on nucleus preservation were also examined. Decalcification with 10% EDTA gave the best result for general cartilage staining. Chondrocyte DNA was retained and intact after using this reagent. Decalcification with 10% EDTA is also the safest method of decalcification if the TUNEL assay is applied to cartilage. Proteinase K digestion may have adverse effects on nucleus preservation in cartilage. Awareness of these effects is important whenever the TUNEL assay is applied." @default.
- W2024458667 created "2016-06-24" @default.
- W2024458667 creator A5044475733 @default.
- W2024458667 creator A5064899156 @default.
- W2024458667 creator A5077652551 @default.
- W2024458667 date "2005-01-01" @default.
- W2024458667 modified "2023-09-27" @default.
- W2024458667 title "The effects of different decalcification protocols on TUNEL and general cartilage staining" @default.
- W2024458667 cites W1605639537 @default.
- W2024458667 cites W2006536993 @default.
- W2024458667 cites W2031592383 @default.
- W2024458667 cites W2046709093 @default.
- W2024458667 cites W2050486464 @default.
- W2024458667 cites W2095168835 @default.
- W2024458667 cites W2138302361 @default.
- W2024458667 cites W2159397083 @default.
- W2024458667 cites W2163193122 @default.
- W2024458667 doi "https://doi.org/10.1080/10520290500159253" @default.
- W2024458667 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16298895" @default.
- W2024458667 hasPublicationYear "2005" @default.
- W2024458667 type Work @default.
- W2024458667 sameAs 2024458667 @default.
- W2024458667 citedByCount "13" @default.
- W2024458667 countsByYear W20244586672013 @default.
- W2024458667 countsByYear W20244586672014 @default.
- W2024458667 countsByYear W20244586672016 @default.
- W2024458667 countsByYear W20244586672018 @default.
- W2024458667 countsByYear W20244586672020 @default.
- W2024458667 countsByYear W20244586672021 @default.
- W2024458667 countsByYear W20244586672023 @default.
- W2024458667 crossrefType "journal-article" @default.
- W2024458667 hasAuthorship W2024458667A5044475733 @default.
- W2024458667 hasAuthorship W2024458667A5064899156 @default.
- W2024458667 hasAuthorship W2024458667A5077652551 @default.
- W2024458667 hasConcept C105702510 @default.
- W2024458667 hasConcept C142724271 @default.
- W2024458667 hasConcept C153911025 @default.
- W2024458667 hasConcept C185592680 @default.
- W2024458667 hasConcept C190283241 @default.
- W2024458667 hasConcept C196795494 @default.
- W2024458667 hasConcept C26905121 @default.
- W2024458667 hasConcept C2779032678 @default.
- W2024458667 hasConcept C2780550940 @default.
- W2024458667 hasConcept C2781403057 @default.
- W2024458667 hasConcept C43617362 @default.
- W2024458667 hasConcept C55493867 @default.
- W2024458667 hasConcept C71924100 @default.
- W2024458667 hasConcept C74864618 @default.
- W2024458667 hasConcept C86803240 @default.
- W2024458667 hasConceptScore W2024458667C105702510 @default.
- W2024458667 hasConceptScore W2024458667C142724271 @default.
- W2024458667 hasConceptScore W2024458667C153911025 @default.
- W2024458667 hasConceptScore W2024458667C185592680 @default.
- W2024458667 hasConceptScore W2024458667C190283241 @default.
- W2024458667 hasConceptScore W2024458667C196795494 @default.
- W2024458667 hasConceptScore W2024458667C26905121 @default.
- W2024458667 hasConceptScore W2024458667C2779032678 @default.
- W2024458667 hasConceptScore W2024458667C2780550940 @default.
- W2024458667 hasConceptScore W2024458667C2781403057 @default.
- W2024458667 hasConceptScore W2024458667C43617362 @default.
- W2024458667 hasConceptScore W2024458667C55493867 @default.
- W2024458667 hasConceptScore W2024458667C71924100 @default.
- W2024458667 hasConceptScore W2024458667C74864618 @default.
- W2024458667 hasConceptScore W2024458667C86803240 @default.
- W2024458667 hasIssue "3-4" @default.
- W2024458667 hasLocation W20244586671 @default.
- W2024458667 hasLocation W20244586672 @default.
- W2024458667 hasOpenAccess W2024458667 @default.
- W2024458667 hasPrimaryLocation W20244586671 @default.
- W2024458667 hasRelatedWork W2015133578 @default.
- W2024458667 hasRelatedWork W2018689368 @default.
- W2024458667 hasRelatedWork W2039545734 @default.
- W2024458667 hasRelatedWork W2363288248 @default.
- W2024458667 hasRelatedWork W2390428747 @default.
- W2024458667 hasRelatedWork W2408944572 @default.
- W2024458667 hasRelatedWork W2409496325 @default.
- W2024458667 hasRelatedWork W2791026082 @default.
- W2024458667 hasRelatedWork W4324351367 @default.
- W2024458667 hasRelatedWork W66484971 @default.
- W2024458667 hasVolume "80" @default.
- W2024458667 isParatext "false" @default.
- W2024458667 isRetracted "false" @default.
- W2024458667 magId "2024458667" @default.
- W2024458667 workType "article" @default.