Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024459032> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2024459032 endingPage "15259" @default.
- W2024459032 startingPage "15257" @default.
- W2024459032 abstract "Mutants of Escherichia coli unable to use fructose by means of the phosphoenolpyruvate/glycose phosphotransferase system mutate further to permit growth on that ketose by derepression of a manno(fructo)kinase (Mak + phenotype) present in only trace amounts in the parent organisms (Mak-o phenotype). The mak gene was located at min 8.8 on the E. coli linkage map as an ORF designated yajF , of hitherto unknown function; it specifies a deduced polypeptide of 344 aa. The derepression of Mak activity was associated with a single base change at position 71 (codon 24) of the gene, where GCC (alanine) in Mak-o has been changed to GAC (aspartate) in Mak + . By cloning selected portions of the total 1,032-bp mak gene into a plasmid that also carried a temperature-sensitive promoter, we showed that the mutation resided in a 117-bp region that does not specify sequences necessary for Mak activity but was located 46 bp upstream of a 915-bp portion that does. Mak + and Mak-o strains differ greatly in the heat stability of the enzyme: at 61°C, mak-o cloned into a mak-o recipient loses 50% of its activity in approximately 6 min, whereas it takes over 30 min to achieve a similar reduction in the activity of mak + cloned into a mak-o strain. However, the Mak activity of the cloned fragment specifying the enzyme without the regulatory region lost activity with a half-life of 29 min irrespective of whether it was derived from a mak + or a mak-o donor, which indicates that the A24D mutation contributes to the high enzyme activity of Mak + mutants by serving to protect Mak from denaturation." @default.
- W2024459032 created "2016-06-24" @default.
- W2024459032 creator A5008764577 @default.
- W2024459032 creator A5017902861 @default.
- W2024459032 creator A5024780477 @default.
- W2024459032 creator A5032672443 @default.
- W2024459032 date "2001-12-11" @default.
- W2024459032 modified "2023-10-06" @default.
- W2024459032 title "Genetic control of manno(fructo)kinase activity in <i>Escherichia coli</i>" @default.
- W2024459032 cites W1489418158 @default.
- W2024459032 cites W1615776902 @default.
- W2024459032 cites W1991499504 @default.
- W2024459032 cites W2012940246 @default.
- W2024459032 cites W2024884021 @default.
- W2024459032 cites W2046244942 @default.
- W2024459032 cites W2059721778 @default.
- W2024459032 cites W2060081863 @default.
- W2024459032 cites W2071955064 @default.
- W2024459032 cites W2102705383 @default.
- W2024459032 cites W2118689526 @default.
- W2024459032 cites W2127819310 @default.
- W2024459032 cites W2134153749 @default.
- W2024459032 cites W2141110578 @default.
- W2024459032 cites W2152859383 @default.
- W2024459032 cites W2169220413 @default.
- W2024459032 doi "https://doi.org/10.1073/pnas.211569798" @default.
- W2024459032 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/65016" @default.
- W2024459032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11742072" @default.
- W2024459032 hasPublicationYear "2001" @default.
- W2024459032 type Work @default.
- W2024459032 sameAs 2024459032 @default.
- W2024459032 citedByCount "16" @default.
- W2024459032 countsByYear W20244590322013 @default.
- W2024459032 countsByYear W20244590322018 @default.
- W2024459032 countsByYear W20244590322020 @default.
- W2024459032 countsByYear W20244590322023 @default.
- W2024459032 crossrefType "journal-article" @default.
- W2024459032 hasAuthorship W2024459032A5008764577 @default.
- W2024459032 hasAuthorship W2024459032A5017902861 @default.
- W2024459032 hasAuthorship W2024459032A5024780477 @default.
- W2024459032 hasAuthorship W2024459032A5032672443 @default.
- W2024459032 hasBestOaLocation W20244590322 @default.
- W2024459032 hasConcept C104317684 @default.
- W2024459032 hasConcept C143065580 @default.
- W2024459032 hasConcept C150194340 @default.
- W2024459032 hasConcept C153911025 @default.
- W2024459032 hasConcept C186310378 @default.
- W2024459032 hasConcept C22744801 @default.
- W2024459032 hasConcept C45656491 @default.
- W2024459032 hasConcept C501734568 @default.
- W2024459032 hasConcept C54355233 @default.
- W2024459032 hasConcept C547475151 @default.
- W2024459032 hasConcept C55493867 @default.
- W2024459032 hasConcept C69034178 @default.
- W2024459032 hasConcept C73766848 @default.
- W2024459032 hasConcept C86803240 @default.
- W2024459032 hasConceptScore W2024459032C104317684 @default.
- W2024459032 hasConceptScore W2024459032C143065580 @default.
- W2024459032 hasConceptScore W2024459032C150194340 @default.
- W2024459032 hasConceptScore W2024459032C153911025 @default.
- W2024459032 hasConceptScore W2024459032C186310378 @default.
- W2024459032 hasConceptScore W2024459032C22744801 @default.
- W2024459032 hasConceptScore W2024459032C45656491 @default.
- W2024459032 hasConceptScore W2024459032C501734568 @default.
- W2024459032 hasConceptScore W2024459032C54355233 @default.
- W2024459032 hasConceptScore W2024459032C547475151 @default.
- W2024459032 hasConceptScore W2024459032C55493867 @default.
- W2024459032 hasConceptScore W2024459032C69034178 @default.
- W2024459032 hasConceptScore W2024459032C73766848 @default.
- W2024459032 hasConceptScore W2024459032C86803240 @default.
- W2024459032 hasIssue "26" @default.
- W2024459032 hasLocation W20244590321 @default.
- W2024459032 hasLocation W20244590322 @default.
- W2024459032 hasLocation W20244590323 @default.
- W2024459032 hasLocation W20244590324 @default.
- W2024459032 hasOpenAccess W2024459032 @default.
- W2024459032 hasPrimaryLocation W20244590321 @default.
- W2024459032 hasRelatedWork W1560279942 @default.
- W2024459032 hasRelatedWork W1571771606 @default.
- W2024459032 hasRelatedWork W1606107581 @default.
- W2024459032 hasRelatedWork W1837225500 @default.
- W2024459032 hasRelatedWork W1873683136 @default.
- W2024459032 hasRelatedWork W2028680712 @default.
- W2024459032 hasRelatedWork W2350652591 @default.
- W2024459032 hasRelatedWork W2372782722 @default.
- W2024459032 hasRelatedWork W2432051187 @default.
- W2024459032 hasRelatedWork W2524850911 @default.
- W2024459032 hasVolume "98" @default.
- W2024459032 isParatext "false" @default.
- W2024459032 isRetracted "false" @default.
- W2024459032 magId "2024459032" @default.
- W2024459032 workType "article" @default.