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• W2024478757 abstract "Any gene can be expressed to some degree in any organism ranging from bacteria, yeast and insects, to transgenic sheep! Specialized vectors have been developed for efficiently introducing DNA into cells and for stably maintaining the DNA either as an episome or integrated into the genome. Transcription and translation signals have also been manipulated to maximize the expression of a heterologous gene in each type of host cell. The choice of host—vector system for the commercial production of mammalian proteins is no longer dictated by the availability of the system but by economic and logistic factors and, in some cases, by the necessity of producing proteins with specific post translational modifications. In this article we discuss the genetic manipulation of yeast (S. cerevisiae) to highlight some of the advantages and disadvantages of this simple eukaryote as a host for the production of mammalian proteins." @default.
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• W2024478757 date "1987-02-01" @default.
• W2024478757 modified "2023-10-15" @default.
• W2024478757 title "The production of mammalian proteins in Saccharomyces cerevisiae" @default.
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• W2024478757 doi "https://doi.org/10.1016/0167-7799(87)90038-2" @default.
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