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• W2024480931 abstract "Nitrate reductase deficient plants of Nicotiana tabacum were unable to utilize ammonium efficiently unless the medium was buffered against excessive acidity accumulation. In addition to succinate, calcium carbonate, MES and phosphate buffers allowed plants to utilize ammonium efficiently. Similar observations were made regarding callus derived from stem tissue of these plants. Plants could be grown in compost, in a physiological state suitable for protoplast isolation, when watered with nutrient solutions containing NH4NO3 and MES buffer. Protoplast division and proliferation to the stage of plant regeneration was possible using Murashige and Skoog (1962) basal medium buffered with succinate, calcium carbonate or MES but not phosphate." @default.
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• W2024480931 date "1986-05-01" @default.
• W2024480931 modified "2023-10-14" @default.
• W2024480931 title "Control of pH and its Effect on Ammonium Utilization by Nitrate Reductase Deficient Plants, Cells, and Protoplasts of Nicotiana tabacum" @default.
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• W2024480931 doi "https://doi.org/10.1016/s0176-1617(86)80089-x" @default.
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