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• W2024482013 endingPage "829" @default.
• W2024482013 startingPage "820" @default.
• W2024482013 abstract "A complete cDNA copy of the fifth RNA segment of the human group B rotavirus, ADRV, has been cloned into plasmid AD512. Gene segment 5 contains 1269 bases and encodes one long open reading frame of 391 amino acids beginning at base 31 and terminating at base 1203. The gene 5 polypeptide, expressed in vitro in a rabbit reticulocyte lysate, comigrates with the 44-kDa major inner capsid protein present on EDTA treated ADRV virions. The gene 5 protein is immunoprecipitable by hyperimmune serum to ADRV, human ADRV convalescent serum and by a group B-specific monoclonal antibody. In addition, this protein shares amino acid identity and similarity with the VP6 proteins from group C and group A rotavirus strains. The ADRV VP6 equivalent protein appears to be more closely related to the group C VP6 than the Group A VP6 polypeptide and a common ancestral rotavirus VP6 precursor protein is suggested. As a result, the fifth RNA segment of ADRV defines the major inner capsid protein, or VP6 equivalent, in the group B rotavirus. Expression of the ADRV VP6 equivalent protein is potentially useful for evaluating the prevalence of serum antibodies to group B rotavirus in human and animal populations as well as for generating antibodies for the direct detection of group B rotavirus antigen." @default.
• W2024482013 created "2016-06-24" @default.
• W2024482013 creator A5018413449 @default.
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• W2024482013 date "1991-06-01" @default.
• W2024482013 modified "2023-09-26" @default.
• W2024482013 title "Expression of the major inner capsid protein of the group B rotavirus ADRV: Primary characterization of genome segment 5 Primary characterization of genome segment 5" @default.
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• W2024482013 doi "https://doi.org/10.1016/0042-6822(91)90623-j" @default.
• W2024482013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1850929" @default.
• W2024482013 hasPublicationYear "1991" @default.
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