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- W2024487282 abstract "Abstract Col E1 DNA can be purified to a high degree by RPC-5 chromatography of a partially purified cell lysate with a very shallow linear NaCl gradient at pH 7.8. Electron micrographs demonstrated that the purest fractions were composed of 93% supercoiled (form I) DNA and 7% open circular (form II) DNA. The actual chromatography can be accomplished in 13–14 h and is designed for the production of several milligrams of plasmid DNA." @default.
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- W2024487282 date "1981-07-01" @default.
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- W2024487282 title "Purification of supercoiled DNA of plasmid Col E1 by RPC-5 chromatography" @default.
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- W2024487282 doi "https://doi.org/10.1016/0003-2697(81)90476-0" @default.
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