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- W2024489583 abstract "The aim of this study was to search for a good inducer agent using for cardiomyogenic differentiation of stem cells. Human placenta-derived mesenchymal stem cells (hPDMSCs) were isolated and incubated in enriched medium. Fourth passaged cells were treated with 10 mg/L dan-shen root for 20 days. Morphologic characteristics were analyzed by confocal and electron microscopy. Expression of α-sarcomeric actin was analyzed by immunohistochemistry. Expression of cardiac troponin-I (TnI) was analyzed by immunohistofluorescence. Atrial natriuretic factor (ANF) and beta-myocin heavy chain (β-MHC) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). hPDMSCs treated with dan-shen root gradually formed a stick-like morphology and connected with adjoining cells. On the 20th day, most of the induced cells stained positive with α-sarcomeric actin and TnI antibody. ANF and β-MHC were also detected in the induced cells. Approximately 80% of the cells were successfully transdifferentiated into cardiomyocytes. In conclusion, dan-shen root is a good inducer agent used for cardiomyogenic differentiation of hPDMSCs." @default.
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- W2024489583 date "2011-11-01" @default.
- W2024489583 modified "2023-09-27" @default.
- W2024489583 title "The effects of dan-shen root on cardiomyogenic differentiation of human placenta-derived mesenchymal stem cells" @default.
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- W2024489583 doi "https://doi.org/10.1016/j.bbrc.2011.10.035" @default.
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